Tailoring hierarchical meso- macroporous 3D scaffolds: from nano to macro



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Cicuéndez Maroto, Mónica and Malmsten, Martin and Doadrio Villarejo, Juan Carlos and Portolés Pérez, M. T. and Izquierdo-Barba, Isabel and Vallet Regí, María (2014) Tailoring hierarchical meso- macroporous 3D scaffolds: from nano to macro. Journal of Materials Chemistry B, 2 . pp. 49-58. ISSN 2050-750X

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Bone tissue regeneration requires the use of 3D scaffolds which mimic the architecture of the natural extracellular matrix, creating an adequate microenvironment for bone cell growth. Such 3D scaffolds need surface properties suitable for biological recognition in the early stage of cell adhesion, necessary to ensure complete cell colonization, retained cell functionality, and subsequently bone regeneration. Herein, hierarchical 3D scaffolds based on new hydroxyapatite/mesoporous glass nanocomposite bioceramic (MGHA) exhibiting different scales of porosity have been synthesized. These 3D scaffolds possess: (i) highly ordered mesopores with diameters of 10 nm; (ii) macropores with diameters in the 30-80 mu m range with interconnections of 1-10 mu m; and (iii) large macropores of ca. 500 mu m. To improve their surface properties, 3D scaffolds were modified through direct functionalization with amine propyl groups, which notably improve preosteoblast adhesion, proliferation (2.3 fold), differentiation (4.8 fold) and further cell colonization of these scaffolds. The observed enhancement can be related to these amine groups which favour early adhesion, e. g., based on nonspecific protein adsorption as was demonstrated by ellipsometry. These results suggest that the combination of hierarchical structure design and amine surface modification of hydroxyapatite/mesoporous nanocomposite scaffolds yields a double increase in cell proliferation, as well as a quadruple increase in cell differentiation, demonstrating the potential of these nanocomposite materials for bone tissue regeneration purposes.

Item Type:Article
Additional Information:

RESEARCHER ID M-3378-2014 (María Vallet Regí)
ORCID 0000-0002-6104-4889 (María Vallet Regí)

Uncontrolled Keywords:Enhanced osteoblast adhesion, Cell -Adhesion, Mesoporous materials, Calcium Phosphates, Delivery-Systems, Bioactive glass, Bone, Surfaces, Design, Hydroxyapatite
Subjects:Sciences > Chemistry > Materials
Sciences > Chemistry > Chemistry, Inorganic
ID Code:41097
Deposited On:09 Feb 2017 12:39
Last Modified:09 Feb 2017 13:03

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