Parathyroid hormone-related protein (107-111) improves the bone regeneration potential of gelatin-glutaraldehyde biopolymer-coated hydroxyapatite



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Lozano Borregón, Daniel and Sánchez Salcedo, Sandra and Portal Núñez, Sergio and Vila, Mercedes and López Herradón, Ana and Ardura, Juan A. and Mulero, Francisca and Gómez Barrena, Enrique and Vallet Regí, María and Esbrit, Pedro (2014) Parathyroid hormone-related protein (107-111) improves the bone regeneration potential of gelatin-glutaraldehyde biopolymer-coated hydroxyapatite. Acta Biomaterialia, 10 (7). pp. 3307-3316. ISSN 1742-7061

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Biopolymer-coated nanocrystalline hydroxyapatite (HA) made as macroporous foams which are degradable and flexible are promising candidates as orthopaedic implants. The C-terminal (107-111) epitope of parathyroid hormone-related protein (PTHrP) exhibits osteogenic properties. The main aim of this study was to evaluate whether PTHrP (107-111) loading into gelatin-glutaraldehyde biopolymer-coated HA (HA(GIu)) scaffolds would produce an optimal biomaterial for tissue engineering applications. HA(GIu) scaffolds with and without PTHrP (107-111) were implanted into a cavitary defect performed in both distal tibial metaphysis of adult rats. Animals were sacrificed after 4 weeks for histological, microcomputerized tomography and gene expression analysis of the callus. At this time, bone healing occurred only in the presence of PTHrP (107-111)-containing HA(Giu) implant, related to an increase in bone volume/tissue volume and trabecular thickness, cortical thickness and gene expression of osteocalcin and vascular cell adhesion molecule I, but a decreased gene expression of Wnt inhibitors, SOST and dickkopf homolog 1. The autonomous osteogenic effect of the PTHrP (107-111)-loaded HA(Giu) scaffolds was confirmed in mouse and human osteoblastic cell cultures. Our findings demonstrate the advantage of loading PTHrP (107-111) into degradable HA(GIu) scaffolds for achieving an optimal biomaterial that is promising for low load bearing clinical applications. (C) 2014 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

Item Type:Article
Additional Information:

RESEARCHER ID M-3378-2014 (María Vallet Regí)
ORCID 0000-0002-6104-4889 (María Vallet Regí)
RESEARCHER ID N-4501-2014 (Sandra Sánchez Salcedo)
ORCID 0000-0002-1889-2057 (Sandra Sánchez Salcedo)
RESEARCHER ID B-5081-2017 (Daniel Lozano Borregón)
ORCID 0000-0001-5902-9201 (Daniel Lozano Borregón)

Uncontrolled Keywords:Macroporous scaffolds, Hydroxyapatite, PTHrP (107-111), In vivo bone regeneration, Rat
Subjects:Sciences > Chemistry > Materials
Sciences > Chemistry > Chemistry, Inorganic
ID Code:41105
Deposited On:13 Feb 2017 12:04
Last Modified:13 Feb 2017 14:55

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