Antigenicity of Anisakis simplex s.s. L3 in parasitized fish after heating conditions used in the canning processing



Downloads per month over past year

Tejada, Margarita and Olivares, Fabiola and de las Heras, Cristina and Careche, Mercedes and Solas Alados, Mª Teresa and García, María Luisa and Fernández, Agustín and Mendizábal, Ángel and Navas, Alfonso and Rodríguez Mahillo, Ana Isabel and González Muñoz, Miguel (2015) Antigenicity of Anisakis simplex s.s. L3 in parasitized fish after heating conditions used in the canning processing. Journal of the Science of Food and Agriculture, 95 . pp. 922-927. ISSN 0022-5142

[thumbnail of Tejada.2015. Antigenicity of Anisakis simplex.pdf] PDF
Restringido a Repository staff only


Official URL:


BACKGROUND: Some technological and food processing treatments applied to parasitized fish kill the Anisakis larvae andprevent infection and sensitization of consumers. However, residual allergenic activity of parasite allergens has been shown.The aim here was to study the effec t of different heat treatments used in the fish canning processing industry on the antigenrecognition of Anisakis L3.Bigeyetuna(Thunnus obesus) and yellowfin tuna (Thunnus albacares) were experimentally infectedwith live L3 Anisakis.After48hat5± 1∘C, brine was added to the muscle, which was then canned raw (live larvae) or heated(90∘C, 30 min) (dead larvae) and treated at 113∘C for 60 min or at 115∘C for 90 min. Anisakis antigens and Ani s 4 were detectedwith anti-crude extract and anti-Ani s 4 antisera respectively.
RESULTS: Ani s 4 decreased in all lots, but the muscle retained part of the allergenicity irrespective of the canning method,as observed by immunohistochemistry. Dot blot analysis showed a high loss of Ani s 4 recognition after canning, but residualantigenicity was present.
CONCLUSION: The results indicate that heat treatment for sterilization under the conditions studied produces a decrease inAni s 4 and suggest a p otential exposure risk for Anisakis-sensitized patients.

Item Type:Article
Uncontrolled Keywords:Anisakis simplex; canning; allergens; Ani s 4; immunohistochemistry; dot blot; Antigens
Subjects:Medical sciences > Biology
Medical sciences > Biology > Microbiology
Medical sciences > Biology > Fishes
ID Code:41942
Deposited On:27 Mar 2017 17:43
Last Modified:28 Mar 2017 08:23

Origin of downloads

Repository Staff Only: item control page