Direct and cross-recognition of lichenized Trebouxia Puymaly (Chlorophyta, Trebouxiophyceae) and Nostoc Vaucher ex Bornet (Cyanobacteria, Cyanophyceae) by their homologous and heterologous fungal lectins

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Díaz Peña, Eva María and Cutrona, Carmen and Sánchez Elordi, Elena and Legaz González, María Estrella and Vicente Córdoba, Carlos (2016) Direct and cross-recognition of lichenized Trebouxia Puymaly (Chlorophyta, Trebouxiophyceae) and Nostoc Vaucher ex Bornet (Cyanobacteria, Cyanophyceae) by their homologous and heterologous fungal lectins. Brazilian Journal of Botany, 39 (2). pp. 507-518. ISSN 0100-8404

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Official URL: https://link.springer.com/journal/40415



Abstract

In this study, we have used affinity chromatography to purify the lectins from a chlorolichen, Evernia prunastri (L.) Ach., and a cyanolichen, Peltigera canina (Ach.) Schard. These species secrete lectins that display arginase activity in addition to their role as recognition proteins. We found that fluorescently labeled lectins display efficient binding to their ligands on the cell wall. Binding was stronger when the lectin reacted with the producing (homologous) photobiont (alga or cyanobacteria) than with the nonproducing (heterologous) species used throughout the study. To address the specificity of lectin binding, we performed desorption experiments of cell-bound lectins with different hexoses. We found that Evernia lectin is only desorbed by galactose, consistent with its specific binding to a single polygalactosylated ligand. Conversely, Peltigera lectin is desorbed not only by galactose, but also by mannose. This indicates that Peltigera lectin recognizes not only α-d-galactose-containing ligands, but also ligands containing α-d-mannose moieties.


Item Type:Article
Uncontrolled Keywords:Arginase; Chlorobiont; Cyanobiont; Lectin; Specificity
Subjects:Medical sciences > Biology
Medical sciences > Biology > Botany
Medical sciences > Biology > Plant physiology
Medical sciences > Biology > Microbiology
ID Code:42151
Deposited On:06 Apr 2017 14:53
Last Modified:09 Apr 2019 09:21

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