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Tissue-specific expression of ghrelinergic and NUCB2/nesfatin-1 systems in goldfish (Carassius auratus) is modulated by macronutrient composition of diets

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Blanco Imperiali, Ayelén M. and Bertucci, Juan I. and Delgado Saavedra, María Jesús and Valenciano González, Ana Isabel and Unniappan, Suraj (2016) Tissue-specific expression of ghrelinergic and NUCB2/nesfatin-1 systems in goldfish (Carassius auratus) is modulated by macronutrient composition of diets. Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology, 195 . pp. 1-9. ISSN 1095-6433

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Official URL: https://www.journals.elsevier.com/comparative-biochemistry-and-physiology-part-a-molecular-and-integrative-physiology



Abstract

The macronutrient composition of diets is a very important factor in the regulation of body weight and metabolism. Several lines of research in mammals have shown that macronutrients differentially regulate metabolic hormones, including ghrelin and nesfatin-1 that have opposing effects on energy balance. This study aimed to determine whether macronutrients modulate the expression of ghrelin and the nucleobindin-2 (NUCB2) encoded nesfatin-1 in goldfish (Carassius auratus). Fish were fed once daily on control, high-carbohydrate, high-protein, high-fat and very high-fat diets for 7 (short-term) or 28 (long-term) days. The expression of preproghrelin, ghrelin O-acyl transferase (goat), growth hormone secretagogue receptor 1 (ghs-r1) and nucb2/nesfatin-1 mRNAs was quantified in the hypothalamus, pituitary, gut and liver. Short-term feeding with fat-enriched diets significantly increased nucb2 mRNA levels in hypothalamus and liver, preproghrelin, goat and ghs-r1 expression in pituitary, and ghs-r1 expression in gut. Fish fed on a high-protein diet exhibited a significant reduction in preproghrelin and ghs-r1 mRNAs in the liver. After long-term feeding, fish fed on high-carbohydrate and very high-fat diets had significantly increased preproghrelin, goat and ghs-r1 expression in pituitary. Feeding on a high-carbohydrate diet also upregulated goat and ghs-r1 transcripts in gut, while feeding on a high-fat diet elicited the same effect only for ghs-r1 in liver. Nucb2 expression increased in pituitary, while it decreased in gut after long-term feeding of a high-protein diet. Collectively, these results show for the first time in fish that macronutrients differentially regulate the expression of ghrelinergic and NUCB2/nesfatin-1 systems in central and peripheral tissues of goldfish.


Item Type:Article
Uncontrolled Keywords:Carbohydrate; Protein; Fat; Ghrelin; GOAT; GHS-R; Nesfatin-1; Fish
Subjects:Medical sciences > Biology
Medical sciences > Biology > Animal physiology
Medical sciences > Biology > Fishes
ID Code:42602
Deposited On:08 May 2017 14:28
Last Modified:14 May 2020 10:07

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