Screening and Identification of Potential Sex-Associated Sequences in Danio rerio



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Luzio, Ana and Coimbra, Ana M. and Benito Jiménez, César and Fontaínhas Fernandes, Antonio A. and Matos, Manuela (2015) Screening and Identification of Potential Sex-Associated Sequences in Danio rerio. Molecular Reproduction and Development, 82 . pp. 756-764. ISSN 1040-452X

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Current knowledge on zebrafish (Danio rerio) suggests that sex determination has a polygenic genetic basis in this species, although environmental factors may also be involved. This study aimed to identify sex-associated genomic regions using two different marker systems: inter-simple sequence repeats (ISSRs) and random-amplified polymorphic DNA (RAPDs). Two bulks were constructed: one with DNA from zebrafish females and the other from males; then, a total of 100 ISSR and 280 RAPD primers were tested. Three DNA fragments presenting sexual dimorphism (female-linked: OPA17436 and OPQ191027 ; male-linked: OPQ19951 ) were determined from sequential analysis of the bulks followed by assessment in individuals. These fragments were cloned and convert into the following sequenced characterized amplified regions (SCAR): DrSM_F1, DrSM_F2, and DrSM_M, which share identities with sequences located in chromosomes 2, 3, and 11 (Zv9), respectively. Using these potential markers in zebrafish samples it was possible to correctly identify 80% of the males (DrSM_M) and 100% of the females (DrSM_F1 + DrSM_F2) in the analyzed population.

Item Type:Article
Uncontrolled Keywords:Cyprinidae ; Danio rerio ; genetics
Subjects:Medical sciences > Biology > Genetics
Medical sciences > Biology > Fishes
ID Code:42867
Deposited On:23 May 2017 11:14
Last Modified:10 Dec 2018 15:30

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