Functional organization of the HIV lipid envelope



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Huarte, Nerea and Carravilla, Pablo and Cruz Rodríguez, Antonio and Lorizate, Maier and Nieto-Garay, Jon A. and Kräusslich, Hans-Georg and Pérez-Gil, Jesús and Requejo Isidro, José and Nieva, José L. (2016) Functional organization of the HIV lipid envelope. Scientific Reports, 6 . pp. 1-14. ISSN 2045-2322, ESSN: 2045-2322

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The chemical composition of the human immunodeficiency virus type 1 (HIV-1) membrane is critical for fusion and entry into target cells, suggesting that preservation of a functional lipid bilayer organization may be required for efficient infection. HIV-1 acquires its envelope from the host cell plasma membrane at sites enriched in raft-type lipids. Furthermore, infectious particles display aminophospholipids on their surface, indicative of dissipation of the inter-leaflet lipid asymmetry metabolically generated at cellular membranes. By combining two-photon excited Laurdan fluorescence imaging and atomic force microscopy, we have obtained unprecedented insights into the phase state of membranes reconstituted from viral lipids (i.e., extracted from infectious HIV-1 particles), established the role played by the different specimens in the mixtures, and characterized the effects of membrane-active virucidal agents on membrane organization. In determining the molecular basis underlying lipid packing and lateral heterogeneity of the HIV-1 membrane, our results may help develop compounds with antiviral activity acting by perturbing the functional organization of the lipid envelope.

Item Type:Article
Uncontrolled Keywords:Chemical biology; Fluorescence imaging; Membrane structure and assembly; Viral membrane fusion
Subjects:Medical sciences > Biology > Biochemistry
ID Code:43222
Deposited On:13 Jun 2017 11:37
Last Modified:31 May 2021 10:15

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