Surfactant dysfunction during over-expression of TGF-β1 precedes profibrotic lung remodeling in vivo

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López Rodríguez, Elena and Boden, Caroline and Echaide Torreguitar, Mercedes and Pérez-Gil, Jesús and Kolb, Martin and Gauldie, Jack and Maus, Ulrich A. and Ochs, Matthias and Knudsen, Lars (2016) Surfactant dysfunction during over-expression of TGF-β1 precedes profibrotic lung remodeling in vivo. American journal of physiology lung cellular and molecular physiology, 310 (11). L1260-L1271. ISSN 1040-0605; Online ISSN: 1522-1504

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Official URL: http://ajplung.physiology.org/content/310/11/L1260



Abstract

Surfactant dysfunction during overexpression of TGF-1 precedes profibrotic lung remodeling in vivo. Am J Physiol Lung Cell Mol Physiol 310: L1260 –L1271, 2016. First published April 22, 2016; doi:10.1152/ajplung.00065.2016.— Transforming growth factor-1 (TGF-1) is involved in regulation of cellular proliferation, differentiation, and fibrogenesis, inducing myofi- broblast migration and increasing extracellular matrix synthesis. Here, TGF-1 effects on pulmonary structure and function were analyzed. Adenovirus-mediated gene transfer of TGF-1 in mice lungs was performed and evaluated by design-based stereology, invasive pulmonary function testing, and detailed analyses of the surfactant system 1 and 2 wk after gene transfer. After 1 wk decreased static compliance was linked with a dramatic alveolar derecruitment without edema formation or increase in the volume of septal wall tissue or collagen fibrils. Abnormally high surface tension correlated with downregulation of surfactant proteins B and C. TTF-1 expression was reduced, and, using PLA (proximity ligand assay) technology, we found Smad3 and TTF-1 forming complexes in vivo, which are normally translocated into the nucleus of the alveolar epithelial type II cells (AE2C) but in the presence of TGF-1 remain in the cytoplasm. AE2C show altered morphology, resulting in loss of total apical surface area per lung and polarity. These changes of AE2C were progressive 2 wk after gene transfer and correlated with lung compliance. Although static lung compliance remained low, the volume of septal wall tissue and collagen fibrils increased 2 wk after gene transfer. In this animal model, the primary effect of TGF-1 signaling in the lung is downregulation of surfactant proteins, high surface tension, alveolar derecruitment, and mechanical stress, which precede fibrotic tissue remodeling and progressive loss of AE2C polarity. Initial TTF-1 dysfunction is potentially linked to downregulation of surfactant proteins. TGF-1


Item Type:Article
Uncontrolled Keywords:TGF-1; TTF-1; AE2C polarity; Surfactant; Pulmonary fibrosis
Subjects:Medical sciences > Biology > Biochemistry
ID Code:43478
Deposited On:27 Jun 2017 11:01
Last Modified:31 May 2021 10:26

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