Targeting conserved genes in fusarium species



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Gil-Serna, Jessica and Patiño Álvarez, Aurora Belén and Jurado, Miguel and Mirete Castañeda, Salvador and Vázquez Estévez, Covadoga and González Jaén, María Teresa (2017) Targeting conserved genes in fusarium species. In Mycotoxigenic Fungi: Methods and Protocols. Methods in Molecular Biology (1542). Springer, pp. 141-147. ISBN 978-1-4939-6705-6 (Print) 978-1-4939-6707-0 (Online)

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Fumonisins are important mycotoxins contaminating foods and feeds which are mainly produced by F. verticillioides and F. proliferatum. Additionally, both are pathogens of maize and other cereals. We describe two highly sensitive, rapid, and species-specific PCR protocols which enable detection and discrimination of these closely related species in cereal flour or grain samples. The specific primer pairs of these assays were based on the intergenic spacer region of the multicopy rDNA unit which highly improves the sensitivity of the PCR assay in comparison with single-copy target regions.

Item Type:Book Section
Uncontrolled Keywords:Fusarium verticillioides; Fusarium proliferatum; Fumonisins; Polymerase chain reaction (PCR); Detection; rDNA intergenic spacer (IGS)
Subjects:Medical sciences > Biology
Medical sciences > Biology > Genetics
Medical sciences > Biology > Microbiology
ID Code:44039
Deposited On:21 Jul 2017 10:42
Last Modified:23 Sep 2020 11:00

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