Functional Analysis of Arabidopsis ARGONAUTEs in Meiosis and DNA Repair



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Martínez García, Marina and Pradillo Orellana, Mónica (2017) Functional Analysis of Arabidopsis ARGONAUTEs in Meiosis and DNA Repair. In Plant Argonaute Proteins: Methods and Protocols. Methods in Molecular Biology (1640). Springer, pp. 145-158. ISBN 978-1-4939-7164-0 (print); 978-1-4939-7165-7 (online)

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Plant ARGONAUTE (AGO) proteins regulate a wide range of cellular and developmental functions. Recent findings highlight their role during homologous recombination, a basic mechanism to repair double-strand DNA lesions (in somatic cells) and programmed DNA breaks (in meiocytes). This chapter contains an exhaustive description of procedures applied to analyze meiotic chromosome behavior (cytogenetic techniques) and DNA repair capacity (genotoxicity assays) in AGO-deficient Arabidopsis thaliana mutants.

Item Type:Book Section
Uncontrolled Keywords:Arabidopsis; ARGONAUTEs; Small RNAs; Chromosomes; DNA repair; FISH; Genotoxic agents; Homologous recombination; Meiosis
Subjects:Medical sciences > Biology > Molecular biology
Medical sciences > Biology > Genetics
ID Code:44043
Deposited On:21 Jul 2017 11:04
Last Modified:10 Dec 2018 15:30

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