Publication: Estudio de la pérdida de heterocigosidad en el brazo corto del cromosoma 3 en los carcinomas de células renales (CCR)
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2017-08-24
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Universidad Complutense de Madrid
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Abstract
En los Carcinomas de células renales (CCR) se han descrito e implicado pérdidas de material genético en diversas regiones del brazo corto del cromosoma 3 (3p). Hemos realizado un análisis de microsatélites en la región 3p14-3p25 para estudiar dichas pérdidas de heterocigosidad y su relación con los antecedentes y hallazgos clínicos, características histopatológicas del tumor así como las respectivas tasas de supervivencia global, supervivencia cáncer específica, supervivencia libre de recidiva local, supervivencia libre de metástasis y supervivencia libre de enfermedad. PACIENTES Y MÉTODOS Realizamos un estudio de cohortes retrospectivo. Se obtuvieron muestras de 41 pacientes sometidos a nefrectomía por tumor renal en el Servicio de Urología del Hospital Clínico San Carlos de Madrid durante los años 1993-1998. Nada más extraer la pieza quirúrgica, se extrajeron dos muestras de tejido renal de cada paciente, una de tejido tumoral y otra de tejido renal sano. Las muestras se congelaron inmediatamente en nitrógeno líquido y después se conservaron a -80ºC hasta su posterior procesamiento. Determinamos el tipo histológico según la clasificación propuesta por WHO en 2004 (3). El grado de diferenciación se realizó siguiendo los criterios de Fuhrman (146)...
In renal cell carcinomas (RCC) have been described and involved loss of genetic material in several regions of the short arm of chromosome 3p. We have made an analysis of microsatellites in the region 3p14-3p25 to study such loss of heterozygosity and its relationship to the history and clinical findings, histopathological characteristics of the tumor and the respective rates of overall survival, cancer-specific survival, local recurrence-free survival, metastasis-free survival and disease-free survival. PATIENTS AND METHODS: We conducted a retrospective cohort study. Samples were taken from 41 patients undergoing nephrectomy for renal tumor in the urology department of San Carlos Clinical Hospital between 1993 to 1998 were obtained. As soon as the surgical specimen was obtained, two samples of kidney tissue of each patient was extracting, one from tumoral tissue and an other from healthy tissue. These samples were immediately frozen in liquid nitrogen and then stored at -80º C until further processing. The histological type was established according to the classification proposed by WHO in 2004 (3). The degree of differentiation was made following Fuhrman criterion (146). The tumor was established according to the TNM classification and stage of the UICC and AJCC 2002 (156)...
In renal cell carcinomas (RCC) have been described and involved loss of genetic material in several regions of the short arm of chromosome 3p. We have made an analysis of microsatellites in the region 3p14-3p25 to study such loss of heterozygosity and its relationship to the history and clinical findings, histopathological characteristics of the tumor and the respective rates of overall survival, cancer-specific survival, local recurrence-free survival, metastasis-free survival and disease-free survival. PATIENTS AND METHODS: We conducted a retrospective cohort study. Samples were taken from 41 patients undergoing nephrectomy for renal tumor in the urology department of San Carlos Clinical Hospital between 1993 to 1998 were obtained. As soon as the surgical specimen was obtained, two samples of kidney tissue of each patient was extracting, one from tumoral tissue and an other from healthy tissue. These samples were immediately frozen in liquid nitrogen and then stored at -80º C until further processing. The histological type was established according to the classification proposed by WHO in 2004 (3). The degree of differentiation was made following Fuhrman criterion (146). The tumor was established according to the TNM classification and stage of the UICC and AJCC 2002 (156)...
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Tesis inédita de la Universidad Complutense de Madrid, Facultad de Medicina, Departamento de Cirugía, leída el 28-01-2016