Label-free quantitative 3D tomographic imaging for partially coherent light microscopy



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Soto Rueda, Juan Manuel and Rodrigo Martín-Romo, José Augusto and Alieva, Tatiana Krasheninnikova (2017) Label-free quantitative 3D tomographic imaging for partially coherent light microscopy. Optics express, 25 (14). pp. 15699-15712. ISSN 1094-4087

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Crucial benefits provided by partially coherent light microscopy such as improved spatial resolution, optical sectioning and speckle-noise suppression are exploited here to achieve 3D quantitative imaging: reconstruction of the object refractive index (RI). We present a partially coherent optical diffraction tomography technique (PC-ODT) that can be easily implemented in commercially available bright-field microscopes. We show that the high numerical apertures of the objective and condenser lenses, together with optical refocusing, are main issues for achieving fast and successful 3D RI reconstruction of weak objects. In particular, the optical refocusing is performed by a high-speed focus tunable lens mounted in front of the digital camera enabling compatibility with commercial microscopes. The technique is experimentally demonstrated on different examples: diatom cells (biosilica shells), polystyrene micro-spheres and blood cells. The results confirm the straightforward 3D-RI reconstruction of the samples providing valuable quantitative information for their analysis. Thus, the PC-ODT can be considered as an efficient and affordable alternative to coherent ODT which requires specially designed holographic microscopes.

Item Type:Article
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© 2017 Optical Society of America.
Funding Ministerio de Economía y Competitividad (TEC2014-57394-P).

Uncontrolled Keywords:Optical diffraction tomography; Phase microscopy; Blood-cells; Resolution
Subjects:Sciences > Physics > Optics
ID Code:45310
Deposited On:02 Nov 2017 12:10
Last Modified:10 Apr 2018 15:10

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