Publication: ¿Cual es la función de las nucleoporinas AtSAR1 y AtSAR3 en la meiosis de " Arabidopsis thaliana"
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2016
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Abstract
El complejo del poro nuclear regula el transporte núcleo-citoplasmático. Existe una relación con la supresión de la resistencia auxinas cuando se alteran algunas nucleoporinas. Concretamente, mutaciones en AtSAR1/AtNUP160 (Suppressor of Auxin Resistance1) y AtSAR3/AtNUP96 suprimen el fenotipo característico de los mutantes Ataxr1 (Auxin Resistant1) como: pérdida de la dominancia apical y del gravitropismo de la raíz, reducción de la formación de raíces laterales, aumento de la longitud de la raíz primaria, reducción de la altura de la planta y de la fertilidad. AtAXR1 es una proteína implicada en la ruta de rubilación. Esta proteína es determinante en la degradación de la proteína Aux/IAA, represora de la expresión de genes de respuesta a auxinas. Además se ha demostrado fundamental durante el proceso de localización de los sobrecruzamientos en la meiosis de Arabidopsis. Los mutantes para las proteínas AtSAR presentaron un fenotipo pleiotrópico, manifestando diversos defectos en el desarrollo vegetativo y la fertilidad. El objetivo del presente trabajo fue caracterizar los mutantes Atsar1-4 y Atsar3-3 para determinar si las alteraciones en fertilidad son debidas a anomalías durante la división meiótica y conocer más sobre las funciones del complejo del poro nuclear.
The nuclear pore complex (NPC) regulates the nucleocytoplasmic transport of macromolecules. Deficiencies in nucleoporins have been found to be involved in the suppression of auxin hormone resistance. Specifically, mutations in SUPPRESSOR OF AUXIN RESISTANCE 1 (AtSAR1/AtNUP160) and AtSAR3 (AtNUP96) suppress many of the phenotypes showed by the Auxin resistant1 (Ataxr1) mutant, namely: defects in root gravitropism, lateral root formation, root hair growth, apical dominance, plant height and fertility. AtAXR1 functions in the RELATED TO UBIQUITIN (RUB) pathway, which is needed for the auxin-dependent degradation of the Aux/IAA proteins that act as transcriptional repressors in auxin signalling. Moreover this pathway is crucial in the crossover location process in Arabidopsis meiosis. Atsar1-4 and Atsar3-3 mutants showed pleiotropic phenotype with multiple growth defects and reduce fertility. The aim of this study was to find whether the reduction in fertility displayed by the Atsar mutants is a consequence of meiotic defects and to have a better understanding of NPC functions in Arabidopsis thaliana.
The nuclear pore complex (NPC) regulates the nucleocytoplasmic transport of macromolecules. Deficiencies in nucleoporins have been found to be involved in the suppression of auxin hormone resistance. Specifically, mutations in SUPPRESSOR OF AUXIN RESISTANCE 1 (AtSAR1/AtNUP160) and AtSAR3 (AtNUP96) suppress many of the phenotypes showed by the Auxin resistant1 (Ataxr1) mutant, namely: defects in root gravitropism, lateral root formation, root hair growth, apical dominance, plant height and fertility. AtAXR1 functions in the RELATED TO UBIQUITIN (RUB) pathway, which is needed for the auxin-dependent degradation of the Aux/IAA proteins that act as transcriptional repressors in auxin signalling. Moreover this pathway is crucial in the crossover location process in Arabidopsis meiosis. Atsar1-4 and Atsar3-3 mutants showed pleiotropic phenotype with multiple growth defects and reduce fertility. The aim of this study was to find whether the reduction in fertility displayed by the Atsar mutants is a consequence of meiotic defects and to have a better understanding of NPC functions in Arabidopsis thaliana.
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Mención Biotecnología Curso 2015-2016