Mesoporous Silica Nanoparticles Functionalized with Hyaluronic Acid. Effect of the Biopolymer Chain Length on Cell Internalization



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Nairi, Valentina and Magnolia, Silvia and Piludu, Marco and Nieddu, Mariella and Caria, Cristian Antonio and Sogos, Valeria and Vallet Regí, María and Monduzzi, Maura and Salis, Andrea (2018) Mesoporous Silica Nanoparticles Functionalized with Hyaluronic Acid. Effect of the Biopolymer Chain Length on Cell Internalization. Colloids and Surfaces B: Biointerfaces . ISSN 0927-7765

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Mesoporous silica nanoparticles (MSNs) were functionalized with amino groups (MSN-NH2) and then with hyaluronic acid, a biocompatible biopolymer which can be recognized by CD44 receptors in tumor cells, to obtain a targeting drug delivery system. To this purpose, three hyaluronic acid samples differing for the molecular weight, namely HAS (8 -15 kDa), HAM (30-50 kDa) and HAL (90-130 kDa), were used. The MSN-HAS, MSN-HAM, and MSN-HAL materials were characterized through zeta potential and dynamic light scattering measurements at pH=7.4 and T=37°C to simulate physiological conditions. While zeta potential showed an increasing negative value with the increase of the HA chain length, an anomalous value of the hydrodynamic diameter was observed for MSN-HAL, which was smaller than that of MSN-HAS and MSN-HAM samples. The cellular uptake of MSN-HA samples on HeLa cells at 37°C was studied by optical and electron microscopy. HA chain length affected significantly the cellular uptake that occurred at a higher extent for MSN-NH2 and MSN-HAS than for MSN-HAM and MSN-HAL samples. Cellular uptake experiments carried out at 4°C showed that the internalization process was inhibited for MSN-HA samples but not for MSN-NH2. This suggests the occurrence of two different mechanisms of internalization. For MSN-NH2 the uptake is mainly driven by the attractive electrostatic interaction with membrane phospholipids, while MSN-HA internalization involves CD44 receptors
overexpressed in HeLa cells.

Item Type:Article
Additional Information:

RESEARCHER ID M-3378-2014 (María Vallet Regí)
ORCID 0000-0002-6104-4889 (María Vallet Regí)

Uncontrolled Keywords:Mesoporous silica nanoparticles, Hyaluronic acid, Cellular uptake, Target system, Optical microscopy, Transmission electron microscopy
Subjects:Sciences > Chemistry > Materials
Medical sciences > Pharmacy > Inorganic chemistry
ID Code:46432
Deposited On:13 Feb 2018 12:01
Last Modified:13 Feb 2020 00:01

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