Unique pharmacological properties of serotoninergic G-protein coupled receptors from cestodes



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Camicia, Federico and Celentano, Ana M. and Johns, Malcolm E. and Chan, John D. and Maldonado, Lucas and Vaca, Hugo and Siervi, Nicolás Di and Kamenetzky, Laura and Gamo Albero, Ana María and Ortega Gutiérrez, Silvia and Martín Fontecha, María del Mar and Davio, Carlos and Marchant, Jonathan S. and Rosenzvit, Mara C. (2018) Unique pharmacological properties of serotoninergic G-protein coupled receptors from cestodes. PLoS ONE, 12 (2). e0006267. ISSN 1932-6203

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Official URL: https://doi.org/10.1371/journal.pntd.0006267


Background: Cestodes are a diverse group of parasites, some of them being agents of neglected diseases. In cestodes, little is known about the functional properties of G protein coupled receptors (GPCRs) which have proved to be highly druggable targets in other organisms. Notably, serotoninergic G-protein coupled receptors (5-HT GPCRs) play major roles in key functions like movement, development and reproduction in parasites.
Methodology/Principal findings: Three 5-HT GPCRs from Echinococcus granulosus and Mesocestoides corti were cloned, sequenced, bioinformatically analyzed and functionally characterized. Multiple sequence alignment with other GPCRs showed the presence of seven transmembrane segments and conserved motifs but interesting differences were also observed. Phylogenetic analysis grouped these new sequences within the 5-HT7 clade of GPCRs. Molecular modeling showed a striking resemblance in the spatial localization of key residues with their mammalian counterparts. Expression analysis using available RNAseq data showed that both E. granulosus sequences are expressed in larval and adult stages. Localization studies performed in E. granulosus larvae with a fluorescent probe produced a punctiform pattern concentrated in suckers. E. granulosus and M. corti larvae showed an increase in motility in response to serotonin. Heterologous expression revealed elevated levels of cAMP production in response to 5-HT and two of the GPCRs showed extremely high sensitivity to 5-HT (picomolar range). While each of these GPCRs was activated by 5-HT, they exhibit distinct pharmacological properties (5-HT sensitivity, differential responsiveness to ligands).
Conclusions/Significance: These data provide the first functional report of GPCRs in parasitic cestodes. The serotoninergic GPCRs characterized here may represent novel druggable targets for antiparasitic intervention.

Item Type:Article
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Uncontrolled Keywords:Cestodes; G protein; Serotonine; GPCRs; 5-HT GPCRs; antiparasitic intervention
Subjects:Sciences > Chemistry > Chemistry, Organic
ID Code:47114
Deposited On:10 Apr 2018 11:53
Last Modified:10 Apr 2018 12:08

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