Effects of a mesoporous bioactive glass on osteoblasts, osteoclasts and macrophages



Downloads per month over past year

Gómez-Cerezo, Natividad and Casarrubios, Laura and Morales, I. and Feito Castellano, María José and Vallet Regí, María and Arcos Navarrete, Daniel and Portolés Pérez, María Teresa (2018) Effects of a mesoporous bioactive glass on osteoblasts, osteoclasts and macrophages. Journal of Colloid and Interface Science, 528 . pp. 309-320. ISSN 0021-9797

[thumbnail of pagination_YJCIS_23677.pdf] PDF

Official URL: https://www.journals.elsevier.com/journal-of-colloid-and-interface-science


A mesoporous bioactive glass (MBG) of molar composition 75SiO2-20CaO-5P2O5 (MBG-75S) has been synthetized as a potential bioceramic for bone regeneration purposes. X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), nitrogen adsorption studies and transmission electron microscopy (TEM) demonstrated that MBG-75S possess a highly ordered mesoporous structure with high surface area and porosity, which would explain the high ionic exchange rate (mainly calcium and silicon soluble species) with the surrounded media. MBG-75S showed high biocompatibility in contact with Saos-2 osteoblast-like cells. Concentrations up to 1 mg/ml did not lead to significant alterations on either morphology or cell cycle. Regarding the effects on osteoclasts, MBG-75S allowed the differentiation of RAW264.7 macrophages into osteoclast-like cells but exhibiting a decreased resorptive activity. These results point out that MBG-75S does not inhibit osteoclastogenesis but reduces the osteoclast bone-resorbing capability. Finally, in vitro studies focused on the innate immune response, evidenced that MBG-75S allows the proliferation of macrophages without inducing their polarization towards the M1 pro-inflammatory phenotype. This in vitro behavior is indicative that MBG-75S would just induce the required innate immune response without further inflammatory complications under in vivo conditions. The overall behavior respect to osteoblasts, osteoclasts and macrophages, makes this MBG a very interesting candidate for bone grafting applications in osteoporotic patients.

Item Type:Article
Additional Information:

RESEARCHER ID M-3378-2014 (María Vallet Regí)
ORCID 0000-0002-6104-4889 (María Vallet Regí)
RESEARCHER ID L-6167-2014 (Daniel Arcos Navarrete)
ORCID 0000-0002-5367-7272 (Daniel Arcos Navarrete)
RESEARCHER ID U-1678-2017 (María Teresa Portolés Pérez)
ORCID 0000-0002-9681-0184 (María Teresa Portolés Pérez)

Uncontrolled Keywords:Mesoporous bioactive glasses, Osteoblasts, Osteoclasts, Macrophages
Subjects:Sciences > Chemistry > Materials
Medical sciences > Pharmacy > Inorganic chemistry
ID Code:47796
Deposited On:08 Jun 2018 09:46
Last Modified:26 Apr 2021 08:11

Origin of downloads

Repository Staff Only: item control page