Publication: Desarrollo de plataformas biosensoras electroquímicas para la detección de marcadores inflamatorios y de cáncer de mama
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2018-08-30
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Universidad Complutense de Madrid
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Abstract
En la actualidad, el diagnóstico clínico demanda la implementación de metodologías de manejo sencillo, coste asequible, reducido tiempo de respuesta y con capacidad de multiplexado que puedan implementarse en la rutina clínica para la determinación de biomarcadores de relevancia en muestras no invasivas, como biopsias líquidas. En respuesta a estas demandas, en esta Tesis Doctoral se ha abordado el desarrollo de varias plataformas biosensoras electroanalíticas aplicables a la determinación de biomarcadores de inflamación y asociados a cáncer de mama. El funcionamiento de la mayoría de estas bioplataformas se basa en la realización de reacciones de inmunoreconocimiento sobre la superficie de micropartículas magnéticas comerciales. Tras su completa modificación, se acoplan sobre electrodos desechables para realizar la transducción amperométrica, cuya magnitud resulta proporcional a la concentración del biomarcador en estudio...
At present the clinical diagnosis demands the implementation of methodologies of simple management, affordable cost, reduced response time and with multiplexing capabilities that can be implemented in the clinical routine for the determination of biomarkers of relevance in non-invasive samples such as liquid biopsies. In response to these demands, this Doctoral Thesis has addressed the development of several electroanalytical biosensing platformsapplicable to the determination ofinflammationbiomarkersandbreast cancer associated biomarkers. The operation of most of these bioplatforms is based on the realization of immunoreactions on the surface of commercial magnetic microparticles that are coupled in the final stage, after their complete modification, on disposable electrodes to performthe amperometric transduction, whose magnitude is proportional to the concentration of the biomarker under study...
At present the clinical diagnosis demands the implementation of methodologies of simple management, affordable cost, reduced response time and with multiplexing capabilities that can be implemented in the clinical routine for the determination of biomarkers of relevance in non-invasive samples such as liquid biopsies. In response to these demands, this Doctoral Thesis has addressed the development of several electroanalytical biosensing platformsapplicable to the determination ofinflammationbiomarkersandbreast cancer associated biomarkers. The operation of most of these bioplatforms is based on the realization of immunoreactions on the surface of commercial magnetic microparticles that are coupled in the final stage, after their complete modification, on disposable electrodes to performthe amperometric transduction, whose magnitude is proportional to the concentration of the biomarker under study...
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Tesis inédita de la Universidad Complutense de Madrid, Facultad de Ciencias Químicas, Departamento de Química Analítica, leída el 19-06-2017