Improved Connective Integration of a Degradable 3D-Nano-Apatite/Agarose Scaffold Subcutaneaosly Implanted in a Rat Model



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García-Honduvilla, Natalio and Coca, Alejandro and Ortega, Miguel A and Trejo, Cynthia and Roma, Jesús and Peña, Juan and Cabañas, M Victoria and Vallet Regí, María and Buja, Julia (2018) Improved Connective Integration of a Degradable 3D-Nano-Apatite/Agarose Scaffold Subcutaneaosly Implanted in a Rat Model. Journal of Biomaterials Applications . ISSN 1530-8022 (In Press)

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In this work, we evaluate the tissue response and tolerance to a designed 3D porous scaffold composed of nanocrystalline carbonate-hydroxyapatite and agarose as a preliminary step in bone repair and regeneration. These scaffolds were subcutaneously implanted into rats, which were sacrificed at different times. CD4þ, CD8þ and ED1þ cells were evaluated as measurements of inflammatory reaction and tolerance. We observed some inflammatory response early after subcutaneous implantation. The 3D interconnected porosity increased scaffold integration via the formation of granulation tissue and the generation of a fibrous capsule around the scaffold. The capsule is initially formed by collagen which progressively invades the scaffold, creating a network that supports the settlement of connective tissue and generating a compact structure. The timing of the appearance of CD4þ and CD8þ cell populations is in agreement with the resolved inflammatory response. The appearance of macrophage activity evidences a slow and gradual degradation activity. Degradation started with the agarose component of the scaffold, but the nano-apatite was kept intact for up to 30 days. Therefore, this apatite/agarose scaffold showed a high capacity for integration by a connective network that stabilizes the scaffold and results in slow nano-apatite degradation. The fundamental properties of the scaffold would provide mechanical support and facilitate bone mobilization, which is of great importance in the masticatory system or large bones.

Item Type:Article
Additional Information:

RESEARCHER ID G-8740-2015 (María Victoria Cabañas Criado)
ORCID 0000-0002-4753-5665 (María Victoria Cabañas Criado)
RESEARCHER ID M-3378-2014 (María Vallet Regí)
ORCID 0000-0002-6104-4889 (María Vallet Regí)

Uncontrolled Keywords:Carbonate-hydroxyapatite, Agarose, Pore architecture, Subcutaneous implant, Tolerance
Subjects:Sciences > Chemistry > Materials
Medical sciences > Pharmacy > Inorganic chemistry
ID Code:49975
Deposited On:20 Nov 2018 11:44
Last Modified:23 Nov 2018 10:43

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