An approach for quantification of platinum distribution in tissues by LA-ICPMS imaging using isotope dilution analysis



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Moraleja, I. and Mena, María Luz and Lázaro, A. and Neumann, B. and Tejedor, A. and Jakubowski, N. and Gómez Gómez, M.Milagros and Esteban Fernández, D. (2018) An approach for quantification of platinum distribution in tissues by LA-ICPMS imaging using isotope dilution analysis. Talanta, 178 . pp. 166-171. ISSN 0039-9140

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Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) has been revealed as a convenient technique for trace elemental imaging in tissue sections, providing elemental 2D distribution at a quantitative level. For quantification purposes, in the last years several approaches have been proposed in the literature such as the use of CRMs or matrix matched standards. The use of Isotope Dilution (ID) for quantification by LA-ICPMS has been also described, being mainly useful for bulk analysis but not feasible for spatial measurements so far. In this work, a quantification method based on ID analysis was developed by printing isotope-enriched inks onto kidney slices from rats treated with antitumoral Pt-based drugs using a commercial ink-jet device, in order to perform an elemental quantification in different areas from bio-images. For the ID experiments 194Pt enriched platinum was used. The methodology was validated by deposition of natural Pt standard droplets with a known amount of Pt onto the surface of a control tissue, where could be quantified even 50 pg of Pt, with recoveries higher than 90%. The amount of Pt present in the whole kidney slices was quantified for cisplatin, carboplatin and oxaliplatin-treated rats. The results obtained were in accordance with those previously reported. The amount of Pt distributed between the medullar and cortical areas was also quantified, observing different behavior for the three drugs.

Item Type:Article
Uncontrolled Keywords:Isotopic dilution LA-ICP-MS Quantification Imaging Kidney Pt-based drugs
Subjects:Sciences > Chemistry > Analytic chemistry
ID Code:55240
Deposited On:09 May 2019 13:56
Last Modified:13 May 2019 08:42

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