Nanomechanical properties of composite protein networks of erythroid membranes at lipid surfaces



Downloads per month over past year

Encinar, Mario and Casado, Santiago and Calzado-Martín, Alicia and Natale, Paolo and San Paulo, Álvaro and Calleja, Montserrat and Vélez, Marisela and Monroy Muñoz, Francisco and López-Montero, Iván (2017) Nanomechanical properties of composite protein networks of erythroid membranes at lipid surfaces. Colloids and Surfaces B: Biointerfaces, 149 . pp. 174-183. ISSN 0927-7765

[thumbnail of Colloids2017.pdf]
Creative Commons Attribution Non-commercial No Derivatives.


Official URL:


Erythrocyte membranes have been particularly useful as a model for studies of membrane structure and mechanics. Native erythroid membranes can be electroformed as giant unilamellar vesicles (eGUVs). In the presence of ATP, the erythroid membrane proteins of eGUVs rearrange into protein networks at the microscale. Here, we present a detailed nanomechanical study of individual protein microfilaments forming the protein networks of eGUVs when spread on supporting surfaces. Using Peak Force tapping Atomic Force Microscopy (PF-AFM) in liquid environment we have obtained the mechanical maps of the composite lipid-protein networks supported on solid surface. In the absence of ATP, the protein pool was characterized by a Young’s Modulus Epool ≈ 5–15 MPa whereas the complex filaments were found softer after protein supramolecular rearrangement; Efil ≈ 0.4 MPa. The observed protein softening and reassembling could be relevant for understanding the mechanisms of cytoskeleton reorganization found in pathological erythrocytes or erythrocytes that are affected by biological agents.

Item Type:Article
Additional Information:

The research leading to these results has received funding from the European Research Council under the European Union's Seventh Framework Programme (ERC grant agreement n° 338133)

Uncontrolled Keywords:Giant vesicles; Erythrocyte; Lipid membrane; Cytoskeleton reconstitution; AFM; PeakForce tapping quantitative nanomechanical mapping
Subjects:Sciences > Physics > Materials
Sciences > Physics > Surfaces (Physics)
ID Code:60110
Deposited On:23 Apr 2020 07:15
Last Modified:23 Apr 2020 07:15

Origin of downloads

Repository Staff Only: item control page