Liquid–liquid phase separation of the Golgi matrix protein GM130



Downloads per month over past year

Rebane, Aleksander A. and Ziltener, Pascal and LaMonica, Lauren C. and Bauer, Antonia H. and Zheng, Hong and López-Montero, Iván and Pincet, Frederic and Rothman, James E. and Ernst, Andreas M. (2020) Liquid–liquid phase separation of the Golgi matrix protein GM130. FEBS Letters, 594 (7). pp. 1132-1144. ISSN 0014-5793

[thumbnail of 1873-3468.13715.pdf]
Creative Commons Attribution.


Official URL:


Golgins are an abundant class of peripheral membrane proteins of the Golgi. These very long (50–400 nm) rod-like proteins initially capture cognate transport vesicles, thus enabling subsequent SNARE-mediated membrane fusion. Here, we explore the hypothesis that in addition to serving as vesicle tethers, Golgins may also possess the capacity to phase separate and, thereby, contribute to the internal organization of the Golgi. GM130 is the most abundant Golgin at the cis Golgi. Remarkably, overexpressed GM130 forms liquid droplets in cells analogous to those described for numerous intrinsically disordered proteins with low complexity sequences, even though GM130 is neither low in complexity nor intrinsically disordered. Virtually pure recombinant GM130 also phase-separates into dynamic, liquid-like droplets in close to physiological buffers and at concentrations similar to its estimated local concentration at the cis Golgi.

Item Type:Article
Additional Information:

The research leading to these results has received funding from the European Research Council under the European Union's Seventh Framework Programme (ERC grant agreement n° 338133)

Uncontrolled Keywords:: coiled-coil; Golgi matrix; Golgin; liquid; liquid phase separation
Subjects:Sciences > Chemistry > Chemistry, Physical and theoretical
Medical sciences > Biology > Molecular biology
Medical sciences > Biology > Biochemistry
ID Code:60126
Deposited On:22 Apr 2020 17:45
Last Modified:23 Apr 2020 10:30

Origin of downloads

Repository Staff Only: item control page