Differential Scanning Calorimetry of Protein–Lipid Interactions



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Cañadas Benito, Olga and Casals Carro, Cristina (2019) Differential Scanning Calorimetry of Protein–Lipid Interactions. In Lipid-Protein Interactions: Methods and Protocols. Methods in Molecular Biology . Humana Press, New York, pp. 91-106. ISBN 9781493995127

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Official URL: https://link.springer.com/protocol/10.1007%2F978-1-4939-9512-7_5


Differential scanning calorimetry (DSC) is a highly sensitive nonperturbing technique used for studying the thermodynamic properties of thermally induced transitions. Since these properties might be affected by ligand binding, DSC is particularly useful for the characterization of protein interactions with biomimetic membranes. The advantages of this technique over other methods consist in the direct measurement of intrinsic thermal properties of the samples, requiring no chemical modifications or extrinsic probes. This chapter describes the basic theory of DSC and provides the reader with an understanding of the capabilities of DSC instrumentation and the type of information that can be achieved from DSC studies of lipid-protein interactions. In particular, the chapter provides a detailed analysis of DSC data to assess the effects of proteins on biomimetic membranes.

Item Type:Book Section
Uncontrolled Keywords:Differential scanning calorimetry, lipids, proteins, lipid–protein interaction, gel to liquid–crystalline phase transition, lamellar to inverted hexagonal phase transition, data analysis
Subjects:Medical sciences > Biology > Biochemistry
ID Code:60710
Deposited On:29 May 2020 16:30
Last Modified:09 Sep 2020 10:17

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