Optimization of a Rabbit Dry Eye Model Induced by Topical Instillation of Benzalkonium Chloride



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Carpena Torres, Carlos and Pintor, Jesús and Pérez de Lara, María Jesús and Huete Toral, Fernando and Crooke, Almudena and Pastrana, Cristina and Carracedo Rodríguez, Juan Gonzalo (2020) Optimization of a Rabbit Dry Eye Model Induced by Topical Instillation of Benzalkonium Chloride. Journal of Ophthalmology, 2020 . 10 p.. ISSN 2090-004X

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Official URL: https://doi.org/10.1155/2020/7204951


Purpose. To optimize a rabbit dry eye model induced by topical instillation of benzalkonium chloride (BAC), reduce the days of instillation of the original model by increasing the concentration of BAC from 0.1% to 0.2%. Materials and Methods. An experimental, prospective, and randomized study was performed on 10 male New Zealand white rabbits, divided into two groups, considering both eyes: 5 rabbits as control (n = 10) and 5 rabbits with 0.2% BAC treatment (n = 10). Saline solution (control) and 0.2% BAC were instilled for 5 consecutive days, twice daily. Tear secretion with and without anesthesia, tear breakup time, tear osmolarity, corneal staining, conjunctival hyperemia, density of goblet cells, height of mucin cloud, and transcript levels of IL-6 were measured before and after the treatment. Results. After the instillation of 0.2% BAC for 5 consecutive days, there was a significant increase in tear secretion without anesthesia (P < 0.001), corneal staining (P < 0.001), conjunctival hyperemia (P < 0.001), and levels of IL-6 mRNA (P = 0.005) compared to the control group. Conversely, there was a decrease in tear secretion with anesthesia (P < 0.001), tear breakup time (P = 0.007), tear osmolarity (P < 0.001), density of goblet cells (P < 0.001), and height of mucin cloud (P < 0.001). Conclusions. )e topical instillation of 0.2% BAC for 5 consecutive days, twice daily, was a proper procedure to induce a rabbit dry eye model, reducing the number of days of instillation compared to the original model (14 days).

Item Type:Article
Additional Information:

This study was published in memory of Jesus Pintor, who passed away on 2 April 2019. This study has been self-funded.

Uncontrolled Keywords:Dry Eye; Rabbit; Topical instillation; Benzalkonium Chloride
Subjects:Medical sciences > Medicine > Ophtalmology
Medical sciences > Optics > Eyes anatomy
Medical sciences > Optics > Physiological optics
ID Code:60780
Deposited On:05 Jun 2020 09:07
Last Modified:05 Jun 2020 09:17

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