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Cuñado Rodríguez, Nieves (2020) Surface Spreading Technique in Plant Meiocytes for Analysis of Synaptonemal Complex by Electron Microscopy. In Plant Meiosis: Methods and Protocols. Methods in Molecular Biology, 2061 . Humana Press, New York, pp. 181-196. ISBN 9781493998180
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Official URL: https://link.springer.com/protocol/10.1007%2F978-1-4939-9512-7_5
Abstract
An improved method of preparing two-dimensional surface spreads of synaptonemal complexes (SCs) in higher plants for examination by electron microscopy is described. This protocol produces clear, well-spread preparations of SCs and unpaired axial cores from a range of meiotic prophase I stages (leptotene to pachytene) from meiocytes of different plant species. Synaptonemal complex (SC) analyses have been widely used in plant cytogenetic studies to address the process of meiotic chromosome synapses, because of the high-resolution allowed by electron microscopy. Although the real role of SC is still enigmatic, its presence and structural conservation in the vast majority of organisms reflect the importance of this protein structure in the meiotic process.
Item Type: | Book Section |
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Uncontrolled Keywords: | Meiosis, synaptonemal complex, surface spreading, silver staining, electron microscopy |
Subjects: | Medical sciences > Biology > Plant physiology Medical sciences > Biology > Genetics Medical sciences > Biology > Microbiology |
ID Code: | 60965 |
Deposited On: | 16 Jun 2020 12:36 |
Last Modified: | 16 Jun 2020 12:36 |
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