Low abundance of NDUFV2 and NDUFS4 subunits of the hydrophilic complex I domain and VDAC1 predicts mammalian longevity



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Mota-Martorell, Natalia and Jové, Mariona and Pradas, Irene and Sánchez, Isabel and Gómez, José and Naudí, Alba and Barja de Quiroga, Gustavo and Pamplona, Reinald (2020) Low abundance of NDUFV2 and NDUFS4 subunits of the hydrophilic complex I domain and VDAC1 predicts mammalian longevity. Redox Biology, 34 (101539). pp. 1-20. ISSN 2213-2317, ESSN: 2213-2317

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Official URL: https://www.sciencedirect.com/science/article/pii/S2213231720303281


Mitochondrial reactive oxygen species (ROS) production, specifically at complex I (Cx I), has been widely suggested to be one of the determinants of species longevity. The present study follows a comparative approach to analyse complex I in heart tissue from 8 mammalian species with a longevity ranging from 3.5 to 46 years. Gene expression and protein content of selected Cx I subunits were analysed using droplet digital PCR (ddPCR) and western blot, respectively. Our results demonstrate: 1) the existence of species-specific differences in gene expression and protein content of Cx I in relation to longevity; 2) the achievement of a longevity phenotype is associated with low protein abundance of subunits NDUFV2 and NDUFS4 from the matrix hydrophilic domain of Cx I; and 3) long-lived mammals show also lower levels of VDAC (voltage-dependent anion channel) amount. These differences could be associated with the lower mitochondrial ROS production and slower aging rate of long-lived animals and, unexpectedly, with a low content of the mitochondrial permeability transition pore in these species.

Item Type:Article
Uncontrolled Keywords:Complex I; Droplet digital PCR; Longevity; Mammals; Mitochondria; NDUFV2 subunit; NDUFS4 subunit; VDAC; Western blot
Subjects:Medical sciences > Biology > Biochemistry
Medical sciences > Biology > Animal physiology
Medical sciences > Biology > Mammals
ID Code:61317
Deposited On:03 Jul 2020 09:47
Last Modified:18 Jul 2022 11:03

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