Multifunctional antibiotic- and zinc-containing mesoporous bioactive glass scaffolds to fight bone infection.

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Heras, Clara and Jiménez Holguín, Javier and Doadrio Villarejo, Antonio Luis and Vallet Regí, María and Sánchez Salcedo, Sandra and Salinas Sánchez, Antonio J. (2020) Multifunctional antibiotic- and zinc-containing mesoporous bioactive glass scaffolds to fight bone infection. Acta Biomaterialia . ISSN 1742-7061 (In Press)

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Abstract

Bone regeneration is a clinical challenge which requires multiple approaches. Sometimes, it also includes the development of osteogenic and antibacterial biomaterials to treat the emergence of possible infection processes arising from surgery. This study evaluates the antibacterial properties of gelatin-coated meso-macroporous scaffolds based on the bioactive glass 80%SiO2–15%CaO–5%P2O5 (mol-%) before (BL-GE) and after being doped with 4% of ZnO (4ZN-GE) and loaded with both saturated and the minimal inhibitory concentrations of one of the antibiotics: levofloxacin (LEVO), vancomycin (VANCO), rifampicin (RIFAM) or gentamicin (GENTA). After physical-chemical characterization of materials, release studies of inorganic ions and antibiotics from the scaffolds were carried out. Moreover, molecular modelling allowed determining the electrostatic potential density maps and the hydrogen bonds of antibiotics and the glass matrix. Antibacterial in vitro studies (in planktonic, inhibition halos and biofilm destruction) with S. aureus and E. coli as bacteria models showed a synergistic effect of zinc ions and antibiotics. The effect was especially noticeable in planktonic cultures of S. aureus with 4ZN-GE scaffolds loaded with VANCO, LEVO or RIFAM and in E. coli cultures with LEVO or GENTA. Moreover, S. aureus biofilms were completely destroyed by 4ZN-GE scaffolds loaded with VANCO, LEVO or RIFAM and the E. coli biofilm total destruction was accomplished with 4ZN-GE scaffolds loaded with GENTA or LEVO. This approach could be an important step in the fight against microbial resistance and provide needed options for bone infection treatment.


Item Type:Article
Additional Information:

RESEARCHER ID D-8709-2017 (Antonio Doadrio Villarejo)
ORCID 0000-0001-8380-4781 (Antonio Doadrio Villarejo)
RESEARCHER ID N-4501-2014 (Sandra Sánchez Salcedo)
ORCID 0000-0002-1889-2057 (Sandra Sánchez Salcedo)
RESEARCHER ID M-3378-2014 (María Vallet Regí)
ORCID 0000-0002-6104-4889 (María Vallet Regí)
RESEARCHER ID M-3316-2014 (Antonio Salinas Sánchez)
ORCID 0000-0002-8408-3389 (Antonio Salinas Sánchez)

Uncontrolled Keywords:Mesoporous bioactive glasses, ZnO, Bone infection, Staphylococcus aureus, Escherichia coli
Subjects:Sciences > Chemistry > Materials
Medical sciences > Pharmacy > Inorganic chemistry
ID Code:61664
Deposited On:28 Jul 2020 08:26
Last Modified:28 Jul 2020 09:29

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