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Ruwisch, Jannik and Sehlmeyer, Kirsten and Roldán López, Nuria and García Álvarez, Begoña and Pérez-Gil, Jesús and Weaver, Timothy E. and Ochs, Matthias and Knudsen, Lars and López-Rodríguez, Elena (2020) Air Space Distension Precedes Spontaneous Fibrotic Remodeling and Impaired Cholesterol Metabolism in the Absence of Surfactant Protein C. American Journal of Respiratory Cell and Molecular Biology, 62 (4). pp. 1-64. ISSN 1535-4989
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Official URL: https://www.atsjournals.org/doi/abs/10.1165/rcmb.2019-0358OC
Abstract
Surfactant protein (SP)-C deficiency is found in samples from patients with idiopathic pulmonary fibrosis, especially in familial forms of this disease. We hypothesized that SP-C may contribute to fibrotic remodeling in aging mice and alveolar lipid homeostasis. For this purpose, we analyzed lung function, alveolar dynamics, lung structure, collagen content, and expression of genes related to lipid and cholesterol metabolism of aging SP-C knockout mice. In addition, in vitro experiments with an alveolar macrophage cell line exposed to lipid vesicles with or without cholesterol and/or SP-C were performed. Alveolar dynamics showed progressive alveolar derecruitment with age and impaired oxygen saturation. Lung structure revealed that decreasing volume density of alveolar spaces was accompanied by increasing of the ductal counterparts. Simultaneously, septal wall thickness steadily increased, and fibrotic wounds appeared in lungs from the age of 50 weeks. This remarkable phenotype is unique to the 129Sv strain, which has an increased absorption of cholesterol, linking the accumulation of cholesterol and the absence of SP-C to a fibrotic remodeling process. The findings of this study suggest that overall loss of SP-C results in an age-dependent, complex, heterogeneous phenotype characterized by a combination of overdistended air spaces and fibrotic wounds that resembles combined emphysema and pulmonary fibrosis in patients with idiopathic pulmonary fibrosis. Addition of SP-C to cholesterol-laden lipid vesicles enhanced the expression of cholesterol metabolism and transport genes in an alveolar macrophage cell line, identifying a potential new lipid–protein axis involved in lung remodeling.
Resumen (otros idiomas)
Este trabajo, realizado en el grupo de Begoña García Álvarez y Jesús Pérez Gil del Dpto. de Bioquímica y Biología Molecular, dentro de la Tesis de Nuria Roldán, es fruto de una colaboración con el grupo de Elena López Rodríguez, quien fue también doctoranda de la Facultad de Biología y es ahora investigadora del Hospital Charité de Berlín. En él se muestra el importante papel de la proteína SP-C del sistema surfactante pulmonar para mantener la homeostasis alveolar durante la mecánica respiratoria. Los ratones KO para SP-C, que no expresan esta proteína, desarrollan estados prefibróticos, con distensión de los espacios aéreos pulmonares y una disfunción de los macrófagos alveolares, cuyo papel en el metabolismo de los lípidos en el pulmón es fundamental.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | surfactant protein C, pulmonary fibrosis, alveolar dynamics, lung structure, alveolar homeostasis, lipid metabolism, alveolar macrophages |
| Subjects: | Medical sciences > Medicine > Pneumology Medical sciences > Biology > Biochemistry |
| ID Code: | 62220 |
| Deposited On: | 21 Sep 2020 11:07 |
| Last Modified: | 01 Feb 2021 23:00 |
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