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The ribotoxin -sarcin can cleave the sarcin/ricin loop on late 60S pre-ribosomes

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Olombrada, Miriam and Peña, Cohue and Rodríguez Galán, Olga and Klingauf Nerurkar, Purnima and Portugal Calisto, Daniela and Oborská Oplová, Michaela and Altvater, Martin and Gavilanes, José G. and Martínez del Pozo, Álvaro and Cruz, Jesús de la and García Ortega, Lucía and Govind Panse, Vikram (2020) The ribotoxin -sarcin can cleave the sarcin/ricin loop on late 60S pre-ribosomes. Nucleic Acids Research, 48 (11). pp. 6210-6222. ISSN 1362-4962

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Official URL: https://doi.org/10.1093/nar/gkaa315




Abstract

The ribotoxin -sarcin belongs to a family of ribonucleases that cleave the sarcin/ricin loop (SRL), a critical functional rRNA element within the large ribosomal subunit (60S), thereby abolishing translation. Whether -sarcin targets the SRL only in mature 60S subunits remains unresolved. Here, we show that, in yeast, -sarcin can cleave SRLs within late 60S pre-ribosomes containing mature 25S rRNA but not nucleolar/nuclear 60S pre-ribosomes containing 27S pre-rRNA in vivo. Conditional expression of -sarcin is lethal, but does not impede early pre-rRNA processing, nuclear export and the cytoplasmic maturation of 60S pre-ribosomes. Thus, SRL-cleaved containing late 60S pre-ribosomes seem to escape cytoplasmic proofreading steps. Polysome analyses revealed that SRL-cleaved 60S ribosomal subunits form 80S initiation complexes, but fail to progress to the step of translation elongation. We suggest that the functional integrity of a -sarcin cleaved SRL might be assessed only during translation.


Item Type:Article
Uncontrolled Keywords:Ribosomes, Peptide Elongation Factor G, Anticodons
Subjects:Sciences > Chemistry > Molecular biology
Sciences > Chemistry > Biochemistry
ID Code:62256
Deposited On:23 Sep 2020 10:18
Last Modified:23 Sep 2020 11:14

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