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Tetrahymena Glutathione peroxidase family: a comparative analysis of these antioxidant enzymes and differential gene expression to metals and Oxidizing Agents

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Cubas-Gaona, Liliana L. and Francisco Martínez, Patricia de and Martín González, Ana María and Gutiérrez Fernández, Juan Carlos (2020) Tetrahymena Glutathione peroxidase family: a comparative analysis of these antioxidant enzymes and differential gene expression to metals and Oxidizing Agents. Microorganisms, 8 (7). pp. 1-23. ISSN ESSN 2076-2607

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Official URL: https://www.mdpi.com/2076-2607/8/7/1008



Abstract

In the present work, an extensive analysis of the putative glutathione peroxidases (GPx) of the eukaryotic microorganism model Tetrahymena thermophila is carried out. A comparative analysis with GPx present in other Tetrahymena species and other very taxonomically diverse ciliates is also performed. A majority of ciliate GPx have replaced the selenocysteine (Sec) by Cys in its catalytic center, so they can be considered as phospholipid hydroperoxide glutathione peroxidases (PHGPx). Selenocysteine insertion sequence (SECIS) elements have been detected in several ciliate GPx that do not incorporate Sec in their amino acid sequences, and conversely, in other ciliate GPx with Sec, no SECIS elements are detected. These anomalies are analyzed and discussed. From the phylogenetic analysis using the ciliate GPx amino acid sequences, the existence of extensive intraand interspecific gene duplications that produced multiple GPx isoforms in each species is inferred. The ancestral character of the selenoproteins is also corroborated. The analysis by qRT-PCR of six selected T. thermophila GPx genes has shown a quantitative differential expression between them, depending on the stressor (oxidizing agents, apoptotic inducer or metals) and the time of exposure.


Item Type:Article
Uncontrolled Keywords:Glutathione peroxidases; Selenocysteine; SECIS elements; Gene expression; Oxidative stress; Tetrahymena
Subjects:Medical sciences > Biology > Genetics
Medical sciences > Biology > Invertebrates
Medical sciences > Biology > Microbiology
ID Code:62539
Deposited On:14 Oct 2020 08:28
Last Modified:02 Jun 2021 07:23

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