Microglial changes in the early aging stage in a healthy retina and an experimental glaucoma model

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Ramírez Sebastián, Ana Isabel and Fernández Albarral, José Antonio and Hoz Montañana, Rosa de and López Cuenca, Inés and García Martín, Elena Salobrar and Rojas Lozano, Pilar and Valiente Soriano, Francisco Javier and Avilés Trigueros, Marcelino and Villegas Pérez, María Paz and Vidal Sanz, Manuel and Triviño Casado, Alberto and Salazar Corral, Juan José and Ramirez Sebastian, Jose Manuel (2020) Microglial changes in the early aging stage in a healthy retina and an experimental glaucoma model. In Glaucoma: A Neurodegenerative Disease of the Retina and Beyond - Part A. Progress in Brain Research, 256 (1). Elsevier, pp. 125-149. ISBN 978-0-12-821106-9

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Official URL: https://doi.org/10.1016/bs.pbr.2020.05.024




Abstract

Glaucoma is an age-related neurodegenerative disease that begins at the onset of aging. In this disease, there is an involvement of the immune system and therefore of the microglia. The purpose of this study is to evaluate the microglial activation using a mouse model of ocular hypertension (OHT) at the onset of aging. For this purpose, we used both naive and ocular hypertensives of 15-month-old mice (early stage of aging). In the latter, we analyzed the OHT eyes and the eyes contralateral to them to compare them with their aged controls. In the eyes of aged naive, aged OHT and aged contralateral eyes, microglial changes were observed compared to the young mice, including: (i) aged naive vs young naive: An increased soma size and vertical processes; (ii) aged OHT eyes vs young OHT eyes: A decrease in the area of the retina occupied by Iba-1 cells and in vertical processes; and (iii) aged contralateral vs young contralateral: A decrease in the soma size and arbor area and an increase in the number of microglia in the outer segment layer. Aged OHT eyes and the eyes contralateral to them showed an up-regulation of the CD68 expression in the branched microglia and a down-regulation in the MHCII and P2RY12 expression with respect to the eyes of young OHT mice. Conclusion: in the early phase of aging, morphological microglial changes along with changes in the expression of MHCII, CD68 and P2RY12, in both naive and OHT mice. These changes appear in aged OHT eyes and the eyes contralateral to them eyes.


Item Type:Book Section
Additional Information:

ISSN: 0079-6123
Available online 16 June 2020.

Uncontrolled Keywords:Microglia; Aging; Glaucoma; Ocular hypertension; Retina; Inflammation; MHCII; CD68; P2RY12; Iba-1; Mouse
Subjects:Medical sciences > Medicine > Biochemistry
Medical sciences > Medicine > Ophtalmology
Medical sciences > Optics > Eyes anatomy
ID Code:62767
Deposited On:12 Nov 2020 13:10
Last Modified:12 Nov 2020 13:45

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