Development of a polarization imaging method to detect paraffin‐embedded pathology tissues before applying other techniques

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Sijilmassi, Ouafa and López Alonso, José Manuel and Río Sevilla, Aurora, del and Barrio Asensio, María del Carmen (2020) Development of a polarization imaging method to detect paraffin‐embedded pathology tissues before applying other techniques. Journal of biophotonics . 11 p.. ISSN 1864-063X

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Official URL: https://doi.org/10.1002/jbio.202000288




Abstract

The present article describes the development of a technique, applied to paraffin‐embedded tissues, which uses three different wavelengths of monochromatic light (λ1 = 445 nm, λ2 = 540 nm and λ3 = 660 nm) for the measures of the degree of polarization, degree of linear polarization, degree of circular polarization and birefringence, all obtained from measurements of Stokes parameters by using polarized light. The goal of this study was to detect changes in developing embryonic mouse eye when pregnant mice fed diets without folic acid for variable periods compared with a healthy control group. We present a biomedical diagnostic technique based on polarized light detection applied to paraffin‐embedded tissues to visualize the structural damage to aid us in the diagnosis before applying other techniques. Through this method, we can visualize and identify which parts of the tissue were altered with respect to the control group.


Item Type:Article
Additional Information:

Received: 17 July 2020; Revised: 6 September 2020; Accepted: 24 September 2020

Uncontrolled Keywords:Biomedical optics; Eye; Imaging; Polarization; Stoles parameters; Tissue Optics
Subjects:Sciences > Physics > Optics
Medical sciences > Optics > Physiological optics
ID Code:62769
Deposited On:03 Nov 2020 16:35
Last Modified:24 Sep 2021 22:00

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