An immunological approach to the biocompatibility of mesoporous SiO2-CaO nanospheres.



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Montes-Casado, María and Sanvicente, Adrián and Casarrubios, Laura and Feito Castellano, María José and Rojo, J.M. and Vallet Regí, María and Arcos Navarrete, Daniel and Portoles, Pilar and Portolés Pérez, María Teresa (2020) An immunological approach to the biocompatibility of mesoporous SiO2-CaO nanospheres. International Journal of Molecular Sciences, 21 . p. 8291. ISSN 1422-0067

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Mesoporous bioactive glass nanospheres (NanoMBGs) have high potential for clinicalapplications. However, the impact of nanoparticles on the immune system needs to be addressed.
In this study, the biocompatibility of SiO2-CaO NanoMBGs was evaluated on different mouse immune cells, including spleen cells subsets, bone marrow-derived dendritic cells (BMDCs), or cell lines likevSR.D10 Th2 CD4+ lymphocytes and DC2.4 dendritic cells. Flow cytometry and confocal microscopy show that the nanoparticles were rapidly and efficiently taken up in vitro by T and B lymphocytes or by specialized antigen-presenting cells (APCs) like dendritic cells (DCs). Nanoparticles were not cytotoxic and had no effect on cell viability or proliferation under T-cell (anti-CD3) or B cell (LPS) stimuli. Besides, NanoMBGs did not affect the balance of spleen cell subsets, or the production of intracellular or secreted pro- and anti-inflammatory cytokines (TNF-α, IFN-γ, IL-2, IL-6, IL-10) by activated T, B, and dendritic cells (DC), as determined by flow cytometry and ELISA. T cell activation surface markers (CD25, CD69 and Induced Costimulator, ICOS) were not altered by NanoMBGs. Maturation of BMDCs or DC2.4 cells in vitro was not altered by NanoMBGs, as shown by expression of Major Histocompatibility Complex (MHC) and costimulatory molecules (CD40, CD80, CD86), or IL-6 secretion. The effect of wortmannin and chlorpromazine indicate a role for phosphoinositide 3-kinase (PI3K), actin and clathrin-dependent pathways in NanoMBG internalization. We thus demonstrate that these NanoMBGs are both non-toxic and non-inflammagenic for murine lymphoid cells and myeloid DCs despite their efficient intake by the cells.

Item Type:Article
Additional Information:

RESEARCHER ID M-3378-2014 (María Vallet Regí)
ORCID 0000-0002-6104-4889 (María Vallet Regí)
RESEARCHER ID L-6167-2014 (Daniel Arcos Navarrete)
ORCID 0000-0002-5367-7272 (Daniel Arcos Navarrete)
RESEARCHER ID U-1678-2017 (María Teresa Portolés Pérez)
ORCID 0000-0002-9681-0184 (María Teresa Portolés Pérez)

Uncontrolled Keywords:Mesoporous bioactive glass; nanomaterial; T cell; B cell; dendritic cell; dendritic cell maturation; phosphoinositide 3-kinase PI3-K; ICOS; endocytosis.
Subjects:Sciences > Chemistry > Materials
ID Code:62964
Deposited On:05 Nov 2020 11:36
Last Modified:26 Apr 2021 08:10

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