Retinal Ganglion Cell Loss and Microglial Activation in a SOD1G93A Mouse Model of Amyotrophic Lateral Sclerosis

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Rojas Lozano, Pilar and Ramírez Sebastián, Ana Isabel and Cadena Santoyo, Manuel and Fernández Arrabal, José A. and García Martín, Elena Salobrar and López Cuenca, Inés and Santos García, Irene and Lago Femia, Eva de and Urcelay Segura, José Luis and Ramirez Sebastian, Jose Manuel and Hoz Montañana, Rosa de and Salazar Corral, Juan José (2021) Retinal Ganglion Cell Loss and Microglial Activation in a SOD1G93A Mouse Model of Amyotrophic Lateral Sclerosis. International journal of molecular science, 22 (4). 20 p.. ISSN 1661-6596, ESSN: 1422-0067

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Official URL: https://doi.org/10.3390/ijms22041663




Abstract

The neurodegenerative disease amyotrophic lateral sclerosis (ALS) affects the spinal cord, brain stem, and cerebral cortex. In this pathology, both neurons and glial cells are affected. However, few studies have analyzed retinal microglia in ALS models. In this study, we quantified the signs of microglial activation and the number of retinal ganglion cells (RGCs) in an SOD1G93A transgenic mouse model at 120 days (advanced stage of the disease) in retinal whole-mounts. For SOD1G93A animals (compared to the wild-type), we found, in microglial cells, (i) a significant increase in the area occupied by each microglial cell in the total area of the retina; (ii) a significant increase in the arbor area in the outer plexiform layer (OPL) inferior sector; (iii) the presence of cells with retracted processes; (iv) areas of cell groupings in some sectors; (v) no significant increase in the number of microglial cells; (vi) the expression of IFN-γ and IL-1β; and (vii) the non-expression of IL-10 and arginase-I. For the RGCs, we found a decrease in their number. In conclusion, in the SOD1G93A model (at 120 days), retinal microglial activation occurred, taking a pro-inflammatory phenotype M1, which affected the OPL and inner retinal layers and could be related to RGC loss


Item Type:Article
Additional Information:

This article belongs to the Special Issue Retinal Neurodegenerative Diseases: Molecular Targets Driving Neuroinflammation and Neuroprotection.
Received: 21 December 2020 / Revised: 1 February 2021 / Accepted: 4 February 2021 / Published: 7 February 2021

Uncontrolled Keywords:microglia; retina; SOD1G93A mouse model; ALS; retinal whole-mount; microglial activation; retinal ganglion cells; pro-inflammatory M1 phenotype; anti-inflammatory M2 phenotype
Subjects:Medical sciences > Medicine > Neurosciences
Medical sciences > Medicine > Ophtalmology
Medical sciences > Optics > Eyes anatomy
ID Code:63964
Deposited On:05 Mar 2021 12:40
Last Modified:05 Mar 2021 13:02

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