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Assessing Autophagy in Archived Tissue or How to Capture Autophagic Flux from a Tissue Snapshot

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Humbert, Magali and Morán, María and de la Cruz-Ojeda, Patricia and Muntané, Jordi and Wiedmer, Tabea and Apostolova, Nadezda and McKenna, Sharon L. and Velasco Díez, Guillermo and Balduini, Walter and Eckhart, Leopold and Janji, Bassam and Sampaio-Marques, Belém and Ludovico, Paula and Žerovnik, Eva and Langer, Rupert and Perren, Aurel and Engedal, Nikolai and Tschan, Mario P. (2020) Assessing Autophagy in Archived Tissue or How to Capture Autophagic Flux from a Tissue Snapshot. Biology, 9 (3). p. 59. ISSN 2079-7737

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Official URL: https://doi.org/10.3390/biology9030059




Abstract

Autophagy is a highly conserved degradation mechanism that is essential for maintaining cellular homeostasis. In human disease, autophagy pathways are frequently deregulated and there is immense interest in targeting autophagy for therapeutic approaches. Accordingly, there is a need to determine autophagic activity in human tissues, an endeavor that is hampered by the fact that autophagy is characterized by the flux of substrates whereas histology informs only about amounts and localization of substrates and regulators at a single timepoint. Despite this challenging task, considerable progress in establishing markers of autophagy has been made in recent years. The importance of establishing clear-cut autophagy markers that can be used for tissue analysis cannot be underestimated. In this review, we attempt to summarize known techniques to quantify autophagy in human tissue and their drawbacks. Furthermore, we provide some recommendations that should be taken into consideration to improve the reliability and the interpretation of autophagy biomarkers in human tissue samples.


Item Type:Article
Uncontrolled Keywords:autophagy; biomarkers; pathology; disease
Subjects:Medical sciences > Biology > Molecular biology
Medical sciences > Biology > Biochemistry
ID Code:64382
Deposited On:16 Mar 2021 16:58
Last Modified:17 Mar 2021 09:13

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