2′-Deoxyribosyltransferase from Bacillus psychrosaccharolyticus: A Mesophilic-Like Biocatalyst for the Synthesis of Modified Nucleosides from a Psychrotolerant Bacterium

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Fresco Taboada, Alba and Fernández Lucas, Jesús and Acebal Sarabia, Carmen and Arroyo Sánchez, Miguel and Ramón, Fernando and Mata Riesco, Isabel de la and Mancheño, José (2018) 2′-Deoxyribosyltransferase from Bacillus psychrosaccharolyticus: A Mesophilic-Like Biocatalyst for the Synthesis of Modified Nucleosides from a Psychrotolerant Bacterium. Catalysts, 8 (1). p. 8. ISSN 2073-4344

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Official URL: https://doi.org/10.3390/catal8010008




Abstract

Structure-function relationships of a novel 20-deoxyribosyltransferase from the psychrotolerant bacterium Bacillus psychrosaccharolyticus (BpNDT) have been exhaustively studied by biochemical and high resolution crystallographic analyses. Despite BpNDT exhibiting some structural features characteristic of cold-adapted enzymes such as localized flexibility in critical loops, its biochemical properties are typical of mesophilic enzymes. BpNDT is a highly symmetrical homohexamer with tightly associated subunits that possesses flexible and short loops bordering the active sites. The catalytic center is essentially identical to that of other mesophilic homologues. Moreover, BpNDT shows that it is a mesophilic-like enzyme since it is not heat-labile and exhibits an apparent unfolding temperature (Tm) of 49 ◦C, being active during 96 h at 40 and 50 ◦C. Finally, BpNDT synthesizes natural and modified nucleosides, with preference for purines as acceptors and pyrimidine nucleosides as donors. Remarkably, the synthesis of several therapeutic nucleosides has been efficiently carried out. In this sense, 5-hydroxymethyl-20 -deoxyuridine (5-HMdUrd), 7-deaza-6-hydroxypurine-20 -deoxyriboside (7-DHPdRib) and theophylline-20 -deoxyriboside were synthesized for the first time by an NDT enzyme, showing the biotechnological interest of BpNDT.


Item Type:Article
Uncontrolled Keywords:20-deoxyribosyltransferase; enzymatic synthesis; oligomeric assembly; protein crystallography; nucleoside analogues; therapeutic nucleosides
Subjects:Medical sciences > Biology > Molecular biology
Medical sciences > Biology > Biochemistry
ID Code:64383
Deposited On:22 Mar 2021 15:22
Last Modified:19 Jul 2022 09:53

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