Quorum Sensing versus Quenching Bacterial Isolates Obtained from MBR Plants Treating Leachates from Municipal Solid Waste

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Soler, Albert and Arregui García-Rovés, Lucía and Arroyo Sánchez, Miguel and Mendoza, José and Muras, Andrea and Álvarez, Cristina and García-Vera, Cristina and Marquina Díaz, Domingo and Santos de la Sen, Antonio and Serrano Barrero, Susana Lourdes (2018) Quorum Sensing versus Quenching Bacterial Isolates Obtained from MBR Plants Treating Leachates from Municipal Solid Waste. International Journal of Environmental Research and Public Health, 15 (5). p. 1019. ISSN 1660-4601

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Official URL: https://doi.org/10.3390/ijerph15051019




Abstract

Quorum sensing (QS) is a mechanism dependent on bacterial density. This coordinated process is mediated by the synthesis and the secretion of signal molecules, called autoinducers (AIs). N-acyl-homoserine lactones (AHLs) are the most common AIs that are used by Gram-negative bacteria and are involved in biofilm formation. Quorum Quenching (QQ) is the interference of QS by producing hydrolyzing enzymes, among other strategies. The main objective of the present study was to identify QS and QQ strains from MBR wastewater treatment plants. A total of 99 strains were isolated from two Spanish plants that were intended to treat leachate from municipal solid waste. Five AHL producers were detected using AHL biosensor strains (Chromobacterium violaceum CV026 and Agrobacterium tumefaciens NT1). Fifteen strains of seventy-one Gram-positive were capable of eliminating or reducing at least one AHL activity. The analysis of 16S rRNA gene sequence showed the importance of the Pseudomonas genus in the production of biofilms and the relevance of the genus Bacillus in the disruption of the QS mechanism, in which the potential activity of lactonase or acylase enzymes was investigated with the aim to contribute to solve biofouling problems and to increase the useful lifespan of membranes.


Item Type:Article
Uncontrolled Keywords:quorum sensing; quorum quenching; AHL; biofouling; MBR; wastewater treatment
Subjects:Medical sciences > Biology > Cytology
Medical sciences > Biology > Molecular biology
ID Code:64390
Deposited On:22 Mar 2021 16:28
Last Modified:22 Mar 2021 17:49

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