Oligonucleotide sensor based on magnetic capture and photoligation of upconverting nanoparticles in solid surfaces



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Méndez González, Diego and Silva Ibáñez, Pedro Pablo and Valiente Dies, Fernando and Gómez Calderón, Óscar and Mendez González, Juan Luis and Laurenti, Marco and Egatz-Gómez, Ana and Díaz García, Elena and Rubio Retama, Jorge and Melle Hernández, Sonia (2021) Oligonucleotide sensor based on magnetic capture and photoligation of upconverting nanoparticles in solid surfaces. Journal of colloid and interface science . 15 p.. ISSN 0021-9797 (In Press)

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Official URL: https://doi.org/10.1016/j.jcis.2021.02.093


In this work, we present a luminescence platform that can be used as point of care system for determining the presence and concentration of specific oligonucleotide sequences. This sensor exhibited a limit of detection as low as 50 fM by means of: i) the use of single-stranded DNA (ssDNA) functionalized magnetic microparticles that captured and concentrated ssDNA-upconverting nanoparticles (ssDNA-UCNPs) on a solid support, when the target sequence (miR-21-5p DNA-analogue) was in the sample, and ii) a photoligation reaction that covalently linked the ssDNA-UCNPs and the ssDNA magnetic microparticles, allowing stringent washes. The presented sensor showed a similar limit of detection when the assays were conducted in samples containing total miRNA extracted from human serum, demonstrating its suitability for detecting small specific oligonucleotide sequences under real-like conditions. The strategy of combining UCNPs, magnetic microparticles, and photoligation reaction provides new insight into low-cost, rapid, and ultra-sensitive detection of oligonucleotide sequences.

Item Type:Article
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Received 6 October 2020, Accepted 21 February 2021, Available online 11 March 2021.

Uncontrolled Keywords:Upconverting nanoparticles; Magnetic microparticles ; DNA detection ; Photoligation ; Magnetic capture ; Magnetic concentration ; Biosensor
Subjects:Sciences > Physics
Sciences > Physics > Optics
Sciences > Physics > Particles
Medical sciences > Medicine > Diagnostic imaging and Nuclear medicine
Medical sciences > Optics > Imaging systems
ID Code:64569
Deposited On:06 Apr 2021 12:09
Last Modified:07 Apr 2021 07:43

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