Overexpression of P2X3 and P2X7 Receptors and TRPV1 Channels in Adrenomedullary Chromaffin Cells in a Rat Model of Neuropathic Pain

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Arribas Blázquez, Marina and Olivos Oré, Luis Alices and Barahona Gomariz, María Victoria and Sánchez de la Muela, Mercedes and Solar, Virginia and Jiménez, Esperanza and Gualix Sánchez, Francisco Javier and McIntosh, J. Michael and Ferrer-Montiel, Antonio and Miras Portugal, María Teresa and Rodríguez Artalejo, Antonio (2019) Overexpression of P2X3 and P2X7 Receptors and TRPV1 Channels in Adrenomedullary Chromaffin Cells in a Rat Model of Neuropathic Pain. International Journal of Molecular Sciences, 20 (1). p. 155. ISSN 1422-0067

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Official URL: https://doi.org/10.3390/ijms20010155




Abstract

We have tested the hypothesis that neuropathic pain acting as a stressor drives functional plasticity in the sympathoadrenal system. The relation between neuropathic pain and adrenal medulla function was studied with behavioral, immunohistochemical and electrophysiological techniques in rats subjected to chronic constriction injury of the sciatic nerve. In slices of the adrenal gland from neuropathic animals, we have evidenced increased cholinergic innervation and spontaneous synaptic activity at the splanchnic nerve–chromaffin cell junction. Likewise, adrenomedullary chromaffin cells displayed enlarged acetylcholine-evoked currents with greater sensitivity to α-conotoxin RgIA, a selective blocker of α9 subunit-containing nicotinic acetylcholine receptors, as well as increased exocytosis triggered by voltage-activated Ca2+ entry. Altogether, these adaptations are expected to facilitate catecholamine output into the bloodstream. Last, but most intriguing, functional and immunohistochemical data indicate that P2X3 and P2X7 purinergic receptors and transient receptor potential vanilloid-1 (TRPV1) channels are overexpressed in chromaffin cells from neuropathic animals. These latter observations are reminiscent of molecular changes characteristic of peripheral sensitization of nociceptors following the lesion of a peripheral nerve, and suggest that similar phenomena can occur in other tissues, potentially contributing to behavioral manifestations of neuropathic pain.


Item Type:Article
Uncontrolled Keywords:P2X3 receptors; P2X7 receptors; TRPV1 channels; α9 nicotinic acetylcholine receptors; neuropathic pain; chromaffin cells; adrenal medulla; stress
Subjects:Medical sciences > Veterinary > Microbiology
Medical sciences > Veterinary > Veterinary pathology
ID Code:64704
Deposited On:19 Apr 2021 15:46
Last Modified:20 Apr 2021 08:30

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