Comparative study of senescent Th biomarkers in healthy donors and early arthritis patients. Analysis of VPAC receptors and their influence

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Villanueva-Romero, Raúl and Lamana, Amalia and Flores Santamaría, Marissa and Carrión Caballo, Mar and Pérez García, Selene and Triguero-Martínez, Ana and Tomero, Eva and Criado, Gabriel and Pablos, José L. and González-Álvaro, Isidoro and Martínez Mora, Carmen and Juarranz Moratilla, Yasmina and Gomáriz, Rosa P. and Gutiérrez Cañas, Irene (2020) Comparative study of senescent Th biomarkers in healthy donors and early arthritis patients. Analysis of VPAC receptors and their influence. Cells, 9 (12). pp. 1-18. ISSN Electronic: 2073-4409

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Official URL: https://doi.org/10.3390/cells9122592




Abstract

Pro-inflammatory CD4+CD28− T cells are characteristic of immunosenescence, but also of several autoimmune/inflammatory diseases. Vasoactive intestinal peptide (VIP) acts as an anti-inflammatory and immunomodulatory mediator on these cells. Our objective was to study the mutual influence between senescent Th cells and VIP axis in early arthritis (EA), comparing with non-EA donors. We characterized the correlation between senescent Th cells and clinic parameters of EA as well as the behavior of senescent Th biomarkers by real-time PCR. Clinical data were systematically recorded at baseline and after 6 months of follow-up. The number of CD4+CD28− T cells measured by sorting is higher in patients who initially meet ACR classification criteria for rheumatoid arthritis (RA) compared to those who were classified as undifferentiated arthritis (UA). A slight positive correlation between EA CD4+CD28− T cells and CRP or ESR and a negative correlation with bone mineral density were found. Th senescent biomarkers in EA CD4+CD28− T cells were similar to donors, however some of them increased after 6 months of follow-up. VPAC receptors were analyzed by real-time PCR and immunofluorescence, and CD4+CD28− T cells showed higher expression of VPAC2 and lower of VPAC1, VPAC2 showing a significant increased expression in EA cells. Sorted CD4+CD28− T cells were in vitro expanded in presence of VIP, wherein VIP increased senescent biomarker CD27, while it diminished CD57 or NKG2 senescent biomarkers. Our study demonstrates for the first time the existence of a link between senescent Th cells and the VIP axis.


Item Type:Article
Uncontrolled Keywords:Senescent Th cells; CD4+CD28− T cells; VPAC receptors; VIP; Early arthritis; Rheumatoid arthritis
Subjects:Medical sciences > Medicine > Immunology
Medical sciences > Medicine > Rheumatology
ID Code:64988
Deposited On:20 Apr 2021 10:53
Last Modified:21 Apr 2021 06:59

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