A Simple Method to Measure Renal Function in Swine by the Plasma Clearance of Iohexol



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Luis-Lima, Sergio and García-Contreras, Consolación and Vázquez Gómez, Marta and Astiz, Susana and Carrara, Fabiola and Gaspari, Flavio and Negrín-Mena, Natalia and Jiménez-Sosa, Alejandro and Jiménez-Hernández, Hugo and González Bulnes, Antonio and Porrini, Esteban (2018) A Simple Method to Measure Renal Function in Swine by the Plasma Clearance of Iohexol. International Journal of Molecular Sciences, 19 (1). p. 232. ISSN 1422-0067

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Official URL: https://doi.org/10.3390/ijms19010232


There is no simple method to measure glomerular filtration rate (GFR) in swine, an established model for studying renal disease. We developed a protocol to measure GFR in conscious swine by using the plasma clearance of iohexol. We used two groups, test and validation, with eight animals each. Ten milliliters of iohexol (6.47 g) was injected into the marginal auricular vein and blood samples (3 mL) were collected from the orbital sinus at different points after injection. GFR was determined using two models: two-compartment (CL2: all samples) and one-compartment (CL1: the last six samples). In the test group, CL1 overestimated CL2 by ~30%: CL2 = 245 ± 93 and CL1 = 308 ± 123 mL/min. This error was corrected by a first-order polynomial quadratic equation to CL1, which was considered the simplified method: SM = −47.909 + (1.176xCL1) − (0.00063968xCL12). The SM showed narrow limits of agreement with CL2, a concordance correlation of 0.97, and a total deviation index of 14.73%. Similar results were obtained for the validation group. This protocol is reliable, reproducible, can be performed in conscious animals, uses a single dose of the marker, and requires a reduced number of samples, and avoids urine collection. Finally, it presents a significant improvement in animal welfare conditions and handling necessities in experimental trials.

Item Type:Article
Uncontrolled Keywords:renal function; iohexol plasma clearance; swine model
Subjects:Medical sciences > Veterinary > Swine
Medical sciences > Veterinary > Animal culture
ID Code:65035
Deposited On:21 Apr 2021 16:20
Last Modified:28 Apr 2021 07:54

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