Physiological Translocation of Lactic Acid Bacteria during Pregnancy Contributes to the Composition of the Milk Microbiota in Mice

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Andrés Leo, Javier de and Jiménez Quintana, Esther Antonia and Chico-Calero, Isabel and Fresno, Manuel and Fernández Álvarez, Leónides and Rodríguez Gómez, Juan Miguel (2017) Physiological Translocation of Lactic Acid Bacteria during Pregnancy Contributes to the Composition of the Milk Microbiota in Mice. Nutrients, 10 (1). p. 14. ISSN 2072-6643

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Official URL: https://doi.org/10.3390/nu10010014




Abstract

The human milk microbiota is a complex and diverse ecosystem that seems to play a relevant role in the mother-to-infant transmission of microorganisms during early life. Bacteria present in human milk may arise from different sources, and recent studies suggest that at least some of them may be originally present in the maternal digestive tract and may reach the mammary gland through an endogenous route during pregnancy and lactation. The objective of this work was to elucidate whether some lactic acid bacteria are able to translocate and colonize the mammary gland and milk. For this purpose, two lactic acid bacteria strains (Lactococcus lactis MG1614 and Lactobacillus salivarius PS2) were transformed with a plasmid containing the lux genes; subsequently, the transformed strains were orally administered to pregnant mice. The murine model allowed the visualization, isolation, and Polymerase Chain Reaction (PCR)-detection of the transformed bacteria in different body locations, including mammary tissue and milk, reinforcing the hypothesis that physiological translocation of maternal bacteria during pregnancy and lactation may contribute to the composition of the mammary and milk microbiota.


Item Type:Article
Uncontrolled Keywords:human milk; translocation; Lactobacillus salivarius; lux; bioluminescence; pregnancy; lactation
Subjects:Medical sciences > Medicine > Gynecology and Obstetrics
ID Code:65105
Deposited On:27 Apr 2021 14:57
Last Modified:28 Apr 2021 08:05

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