Bacteriological and Immunological Profiling of Meconium and Fecal Samples from Preterm Infants: A Two-Year Follow-Up Study

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Gómez, Marta and Moles, Laura and Espinosa-Martos, Irene and Bustos, Gerardo and de Vos, Willem and Fernández Álvarez, Leónides and Rodríguez Gómez, Juan Miguel and Fuentes, Susana and Jiménez Quintana, Esther Antonia (2017) Bacteriological and Immunological Profiling of Meconium and Fecal Samples from Preterm Infants: A Two-Year Follow-Up Study. Nutrients, 9 (12). p. 1293. ISSN 2072-6643

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Official URL: https://doi.org/10.3390/nu9121293




Abstract

An abnormal colonization pattern of the preterm gut may affect immune maturation and exert a long-term influence on the intestinal bacterial composition and host health. However, follow-up studies assessing the evolution of the fecal microbiota of infants that were born preterm are very scarce. In this work, the bacterial compositions of fecal samples, obtained from sixteen 2-year-old infants were evaluated using a phylogenetic microarray; subsequently, the results were compared with those obtained in a previous study from samples of meconium and feces collected from the same infants while they stayed in the neonatal intensive care unit (NICU). In parallel, the concentration of a wide range of cytokines, chemokines, growth factors and immunoglobulins were determined in meconium and fecal samples. Globally, a higher bacterial diversity and a lower interindividual variability were observed in 2-year-olds’ feces, when compared to the samples obtained during their first days of life. Hospital-associated fecal bacteria, that were dominant during the NICU stay, seemed to be replaced, two years later, by genera, which are usually predominant in the healthy adult microbiome. The immune profile of the meconium and fecal samples differed, depending on the sampling time, showing different immune maturation statuses of the gut.


Item Type:Article
Uncontrolled Keywords:prematurity; infant gut microbiota; DNA microarray; immune maturation
Subjects:Medical sciences > Medicine > Gynecology and Obstetrics
Medical sciences > Medicine > Medical microbiology
ID Code:65106
Deposited On:27 Apr 2021 14:43
Last Modified:28 Apr 2021 08:07

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