Role of pulmonary surfactant protein Sp-C dimerization on membrane fragmentation: an emergent mechanism involved in lung defense and homeostasis

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Barriga Torrejón, Alejandro and Morán Lalangui, Michelle and Castillo-Sánchez, José Carlos and Mingarro, Ismael and Pérez-Gil, Jesús and García-Álvarez, Begoña (2021) Role of pulmonary surfactant protein Sp-C dimerization on membrane fragmentation: an emergent mechanism involved in lung defense and homeostasis. BBA - Biomembranes, 1863 (6). pp. 1-11. ISSN 0005-2736

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Official URL: https://doi.org/10.1016/j.bbamem.2021.183572



Abstract

Surfactant protein C (SP-C) is a protein present in the pulmonary surfactant system that is involved in the biophysical properties of this lipoprotein complex, but it also has a role in lung defense and homeostasis. In this article, we propose that the link between both functions could rely on the ability of SP-C to induce fragmentation of phospholipid membranes and generate small vesicles that serve as support to present different ligands to cells in the lungs. Our results using bimolecular fluorescence complementation and tunable resistive pulse sensing setups suggest that SP-C oligomerization could be the triggering event that causes membrane budding and nanovesiculation. As shown by fluorescence microscopy and flow cytometry, these vesicles are differentially assimilated by alveolar macrophages and alveolar type II cells, indicating distinct roles of these alveoli-resident cells in the processing of the SP-C- induced vesicles and their cargo. These results depict a more accurate picture of the mechanisms of this protein, which could be relevant for the comprehension of pulmonary pathologies and the development of new therapeutic approaches.


Item Type:Article
Uncontrolled Keywords:Membrane proteins; Protein-lipid interactions; Cell biology; Pulmonary surfactant; Biophysics; Structural biology
Subjects:Medical sciences > Biology > Molecular biology
Medical sciences > Biology > Biochemistry
ID Code:65826
Deposited On:31 May 2021 07:48
Last Modified:27 Sep 2021 15:40

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