Effect of Pharmacological Inhibition of the Catalytic Activity of Phosphatases of Regenerating Liver in Early T Cell Receptor Signaling Dynamics and IL-2 Production

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Aguilar Sopeña, Óscar and Hernández Pérez, Sara and Alegre Gómez, Sergio and Castro Sánchez, Patricia and Iglesias Ceacero, Alba and Lazo, John S. and Roda Navarro, Pedro (2020) Effect of Pharmacological Inhibition of the Catalytic Activity of Phosphatases of Regenerating Liver in Early T Cell Receptor Signaling Dynamics and IL-2 Production. International Journal of Molecular Sciences, 21 (7). p. 2530. ISSN 1422-0067

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Official URL: https://doi.org/10.3390/ijms21072530




Abstract

We have previously shown the delivery of phosphatase of regenerating liver-1 (PRL-1) to the immunological synapse (IS) and proposed a regulatory role of the catalytic activity of PRLs (PRL-1, PRL-2 and PRL-3) in antigen-induced IL-2 production. Nonetheless, the expression in T cells and delivery to the IS of the highly homologous PRL-3, as well as the role of the catalytic activity of PRLs in antigen-induced early signaling, has not been investigated. Here, the expression of PRL-3 protein was detected in primary CD4 T cells and in the CD4 T cell line Jurkat (JK), in which an overexpressed GFP-PRL-3 fluorescent fusion protein trafficked through the endosomal recycling compartment and co-localized with PLCγ1 signaling sites at the IS. Pharmacological inhibition was used to compare the role of the catalytic activity of PRLs in antigen-induced early signaling and late IL-2 production. Although the phosphatase activity of PRLs was not critical for early signaling triggered by antigen, it seemed to regulate signaling dynamics and was necessary for proper IL-2 production. We propose that enzymatic activity of PRLs has a higher significance for cytokine production than for early signaling at the IS. However, further research will be necessary to deeply understand the regulatory role of PRLs during lymphocyte activation and effector function.


Item Type:Article
Uncontrolled Keywords:immunological synapse; endosomal compartment; phosphatase of regenerating liver; TCR early signaling; cytokine production
Subjects:Medical sciences > Medicine > Immunology
Medical sciences > Medicine > Medical microbiology
ID Code:65884
Deposited On:08 Jun 2021 16:02
Last Modified:08 Jun 2021 16:02

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