Impact of Vitamin D Deficit on the Rat Gut Microbiome



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Robles-Vera, Iñaki and Callejo Arranz, María and Ramos, Ricardo and Duarte, Juan and Pérez Vizcaíno, Francisco (2019) Impact of Vitamin D Deficit on the Rat Gut Microbiome. Nutrients, 11 (11). p. 2564. ISSN 2072-6643

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Inadequate immunologic, metabolic and cardiovascular homeostasis has been related to either an alteration of the gut microbiota or to vitamin D deficiency. We analyzed whether vitamin D deficiency alters rat gut microbiota. Male Wistar rats were fed a standard or a vitamin D-free diet for seven weeks. The microbiome composition was determined in fecal samples by 16S rRNA gene sequencing. The vitamin D-free diet produced mild changes on α- diversity but no effect on β-diversity in the global microbiome. Markers of gut dysbiosis like Firmicutes-to-Bacteroidetes ratio or the short chain fatty acid producing bacterial genera were not significantly affected by vitamin D deficiency. Notably, there was an increase in the relative abundance of the Enterobacteriaceae, with significant rises in its associated genera Escherichia, Candidatus blochmannia and Enterobacter in vitamin D deficient rats. Prevotella and Actinomyces were also increased and Odoribacteraceae and its genus Butyricimonas were decreased in rats with vitamin D-free diet. In conclusion, vitamin D deficit does not induce gut dysbiosis but produces some specific changes in bacterial taxa, which may play a pathophysiological role in the immunologic dysregulation associated with this hypovitaminosis.

Item Type:Article
Uncontrolled Keywords:microbiota; 16S rRNA sequencing; vitamin D deficit
Subjects:Medical sciences > Medicine > Medical microbiology
Medical sciences > Medicine > Toxicology
Medical sciences > Veterinary > Laboratory animals
ID Code:65906
Deposited On:02 Jun 2021 14:15
Last Modified:03 Jun 2021 06:30

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