An Electrochemical Enzyme Biosensor for 3-Hydroxybutyrate Detection Using Screen-Printed Electrodes Modified by Reduced Graphene Oxide and Thionine

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Martínez García, Gonzalo and Pérez Julián, Elena and Agüí Chicharro, María Lourdes and Cabré, Naomí and Joven, Jorge and Yáñez Sedeño, Paloma and Pingarrón Carrazón, José Manuel (2017) An Electrochemical Enzyme Biosensor for 3-Hydroxybutyrate Detection Using Screen-Printed Electrodes Modified by Reduced Graphene Oxide and Thionine. Biosensors, 7 (4). p. 50. ISSN 2079-6374

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Official URL: https://doi.org/10.3390/bios7040050




Abstract

A biosensor for 3-hydroxybutyrate (3-HB) involving immobilization of the enzyme 3-hydroxybutyrate dehydrogenase onto a screen-printed carbon electrode modified with reduced graphene oxide (GO) and thionine (THI) is reported here. After addition of 3-hydroxybutyrate or the sample in the presence of NAD+ cofactor, the generated NADH could be detected amperometrically at 0.0 V vs. Ag pseudo reference electrode. Under the optimized experimental conditions, a calibration plot for 3-HB was constructed showing a wide linear range between 0.010 and 0.400 mM 3-HB which covers the clinically relevant levels for diluted serum samples. In addition, a limit of detection of 1.0 µM, much lower than that reported using other biosensors, was achieved. The analytical usefulness of the developed biosensor was demonstrated via application to spiked serum samples.


Item Type:Article
Uncontrolled Keywords:3-hydroxybutyrate; graphene; thionine; electrochemical biosensor; diabetic ketoacidosis
Subjects:Sciences > Chemistry > Biochemistry
Sciences > Chemistry > Analytic chemistry
ID Code:66142
Deposited On:16 Jun 2021 14:27
Last Modified:17 Jun 2021 07:21

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