Opportunities, Challenges, and Prospects in Electrochemical Biosensing of Circulating Tumor DNA and its Specific Features

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Campuzano Ruiz, Susana and Serafín González-Carrato, Verónica and Gamella Carballo, Maria and Pedrero Muñoz, María and Yáñez Sedeño, Paloma and Pingarrón Carrazón, José Manuel (2019) Opportunities, Challenges, and Prospects in Electrochemical Biosensing of Circulating Tumor DNA and its Specific Features. Sensors, 19 (17). p. 3762. ISSN 1424-8220

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Official URL: https://doi.org/10.3390/s19173762




Abstract

Nowadays, analyzing circulating tumor DNA (ctDNA), a very small part of circulating free DNA (cfDNA) carried by blood, is considered to be an interesting alternative to conventional single-site tumor tissue biopsies, both to assess tumor burden and provide a more comprehensive snapshot of the time-related and spatial heterogeneity of cancer genetic/epigenetic scenery. The determination of ctDNA and/or mapping its characteristic features, including tumor-specific mutations, chromosomal aberrations, microsatellite alterations, and epigenetic changes, are minimally invasive, powerful and credible biomarkers for early diagnosis, follow-up, prediction of therapy response/resistance, relapse monitoring, and tracking the rise of new mutant subclones, leading to improved cancer outcomes This review provides an outline of advances published in the last five years in electrochemical biosensing of ctDNA and surrogate markers. It emphasizes those strategies that have been successfully applied to real clinical samples. It highlights the unique opportunities they offer to shift the focus of cancer patient management methods from actual decision making, based on clinic-pathological features, to biomarker-driven treatment strategies, based on genotypes and customized targeted therapies. Also highlighted are the unmet hurdles and future key points to guide these devices in the development of liquid biopsy cornerstone tools in routine clinical practice for the diagnosis, prognosis, and therapy response monitoring in cancer patients.


Item Type:Article
Uncontrolled Keywords:electrochemical biosensor; circulating tumor DNA; cancer; mutations; epigenetic changes; liquid biopsy
Subjects:Sciences > Chemistry > Analytic chemistry
ID Code:66798
Deposited On:06 Jul 2021 13:56
Last Modified:07 Jul 2021 06:46

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