Hyaluronic Acid Combined with Serum Rich in Growth Factors in Corneal Epithelial Defects



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Suárez-Barrio, Carlota and Etxebarria, Jaime and Hernáez-Moya, Raquel and del Val-Alonso, Marina and Rodriguez-Astigarraga, Maddalen and Urkaregi, Arantza and Freire, Vanesa and Morales, María-Celia and Durán, Juan and Vicario de la Torre, Marta and Molina Martinez, Irene Teresa and Herrero Vanrell, Rocío and Andollo, Noelia (2019) Hyaluronic Acid Combined with Serum Rich in Growth Factors in Corneal Epithelial Defects. International Journal of Molecular Sciences, 20 (7). p. 1655. ISSN 1422-0067

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Official URL: https://doi.org/10.3390/ijms20071655


The aim of this study is to assess if an adhesive biopolymer, sodium hyaluronate (NaHA), has synergistic effects with s-PRGF (a serum derived from plasma rich in growth factors and a blood derivative that has already shown efficacy in corneal epithelial wound healing), to reduce time of healing or posology. In vitro proliferation and migration studies, both in human corneal epithelial (HCE) cells and in rabbit primary corneal epithelial (RPCE) cultures, were carried out. In addition, we performed studies of corneal wound healing in vivo in rabbits treated with s-PRGF, NaHA, or the combination of both. We performed immunohistochemistry techniques (CK3, CK15, Ki67, ß4 integrin, ZO-1, α-SMA) in rabbit corneas 7 and 30 days after a surgically induced epithelial defect. In vitro results show that the combination of NaHA and s-PRGF offers the worst proliferation rates in both HCE and RPCE cells. Addition of NaHA to s-PRGF diminishes the re-epithelializing capability of s-PRGF. In vivo, all treatments, given twice a day, showed equivalent efficacy in corneal epithelial healing. We conclude that the combined use of s-PRGF and HaNA as an adhesive biopolymer does not improve the efficacy of s-PRGF alone in the wound healing of corneal epithelial defects.

Item Type:Article
Uncontrolled Keywords:corneal epithelial defect; cornea regeneration; serum eye drops; plasma rich plasma (PRP); serum derived from plasma rich in growth factors (s-PRGF); hyaluronic acid (NaHA); wound healing
Subjects:Medical sciences > Pharmacy > Pharmacology
Medical sciences > Pharmacy > Drugs
ID Code:67121
Deposited On:21 Jul 2021 14:59
Last Modified:17 Aug 2021 08:43

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