The Subgingival Microbiome in Patients with Down Syndrome and Periodontitis



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Nóvoa, Lourdes and Sánchez, María del Carmen and Blanco, Juan and Limeres, Jacobo and Cuenca, Maigualida and Marín Cuenda, María José and Sanz Alonso, Mariano and Herrera González, David and Diz, Pedro (2020) The Subgingival Microbiome in Patients with Down Syndrome and Periodontitis. Journal of Clinical Medicine, 9 (8). p. 2482. ISSN 2077-0383

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Objective: To describe the subgingival microbiome of individuals with Down syndrome (DS).
Methods: We conducted a cross-sectional observational study that obtained bacterial DNA samples from 50 patients with DS, 25 with periodontitis (PDS) and 25 with a healthy periodontal condition (HDS). The samples were analyzed by sequencing the 16S rRNA gene V3–V4 hypervariable region using the MiSeq System. Taxonomic affiliations were assigned using the naïve Bayesian classifier integrated in QIIME2 plugins. We evaluated the difference in bacteria abundance between the sample groups using Wilcoxon and Kruskal–Wallis tests. We evaluated the alpha diversity of the identified species using the Observed, Chao1metric, ACE and Shannon indices and evaluated beta diversity with principal coordinate analysis (registration code: 2018/510).
Results: Twenty-one genera and 39 bacterial species showed a significantly different abundance between the study groups. Among the genera, Porphyromonas, Treponema, Tannerella and Aggregatibacter were more abundant in the PDS group than in the HDS group, as were the less commonly studied Filifactor, Fretibacterium and Desulfobulbus genera. Among the species, Porphyromonas spp. and Tannerella spp. were the most abundant in the PDS group; the most abundant species in the HDS group were Pseudomonas spp., Granulicatella spp. and Gemella spp.
Conclusion: Well-recognized periodontal pathogens and newly proposed pathogenic taxa were associated with periodontitis in patients with DS.

Item Type:Article
Uncontrolled Keywords:Down syndrome; periodontitis; microbiome; microbial community; DNA sequencing
Subjects:Medical sciences > Dentistry
Medical sciences > Dentistry > Periodontics
ID Code:67497
Deposited On:25 Aug 2021 07:45
Last Modified:25 Aug 2021 07:46

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