Soluble St2 Induces Cardiac Fibroblast Activation and Collagen Synthesis via Neuropilin-1

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Matilla, Lara and Arrieta, Vanessa and Jover, Eva and García-Peña, Amaia and Martínez Martínez, Ernesto and Sadaba, Rafael and Álvarez, Virginia and Navarro, Adela and Fernández-Celis, Amaya and Gainza, Alicia and Santamaria, Enrique and Fernandez Irigoyen, Joaquín and Rossignol, Patrick and Zannad, Faiez and López Andrés, Natalia (2020) Soluble St2 Induces Cardiac Fibroblast Activation and Collagen Synthesis via Neuropilin-1. Cells, 9 (7). p. 1667. ISSN 2073-4409

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Official URL: https://doi.org/10.3390/cells9071667




Abstract

Circulating levels of soluble interleukin 1 receptor-like 1 (sST2) are increased in heart failure and associated with poor outcome, likely because of the activation of inflammation and fibrosis. We investigated the pathogenic role of sST2 as an inductor of cardiac fibroblasts activation and collagen synthesis. The effects of sST2 on human cardiac fibroblasts was assessed using proteomics and immunodetection approaches to evidence the upregulation of neuropilin-1 (NRP-1), a regulator of the profibrotic transforming growth factor (TGF)-β1. In parallel, sST2 increased fibroblast activation, collagen and fibrosis mediators. Pharmacological inhibition of nuclear factor-kappa B (NF-κB) restored NRP-1 levels and blocked profibrotic effects induced by sST2. In NRP-1 knockdown cells, sST2 failed to induce fibroblast activation and collagen synthesis. Exogenous NRP-1 enhanced cardiac fibroblast activation and collagen synthesis via NF-κB. In a pressure overload rat model, sST2 was elevated in association with cardiac fibrosis and was positively correlated with NRP-1 expression. Our study shows that sST2 induces human cardiac fibroblasts activation, as well as the synthesis of collagen and profibrotic molecules. These effects are mediated by NRP-1. The blockade of NF-κB restored NRP-1 expression, improving the profibrotic status induced by sST2. These results show a new pathogenic role for sST2 and its mediator, NRP-1, as cardiac fibroblast activators contributing to cardiac fibrosis.


Item Type:Article
Uncontrolled Keywords:sST2; neuropilin-1; collagen; fibroblast activation; NF-κB
Subjects:Medical sciences > Medicine > Cardiology
Medical sciences > Medicine > Physiology
ID Code:67529
Deposited On:27 Aug 2021 07:16
Last Modified:06 Sep 2021 09:17

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